MgF(3)(-) as a transition state analog of phosphoryl transfer

Debbie L Graham; Peter N Lowe; Geoffrey W Grime; Michael Marsh; Katrin Rittinger; Stephen J Smerdon; Steven J Gamblin; John F Eccleston; Debbie Cunningham

MgF(3)(-) as a transition state analog of phosphoryl transfer

Debbie L Graham, Peter N Lowe, Geoffrey W Grime, Michael Marsh, Katrin Rittinger, Stephen J Smerdon, Steven J Gamblin, John F Eccleston, Debbie Cunningham

Biosciences

Research output: Contribution to journal › Article › peer-review

80 Citations (Scopus)

Abstract

The formation of complexes between small G proteins and certain of their effectors can be facilitated by aluminum fluorides. Solution studies suggest that magnesium may be able to replace aluminum in such complexes. We have determined the crystal structure of RhoA.GDP bound to RhoGAP in the presence of Mg(2+) and F(-) but without Al(3+). The metallofluoride adopts a trigonal planar arrangement instead of the square planar structure of AlF(4)(-). We have confirmed that these crystals contain magnesium and not aluminum by proton-induced X-ray emission spectroscopy. The structure adopted by GDP.MgF(-) possesses the stereochemistry and approximate charge expected for the transition state. We suggest that MgF3(-) may be the reagent of choice for studying phosphoryl transfer reactions.

Original language	English
Pages (from-to)	375-81
Number of pages	7
Journal	Chemistry & Biology
Volume	9
Issue number	3
Publication status	Published - Mar 2002

Keywords

Aluminum Compounds
Fluorides
Guanosine Diphosphate
Magnesium Compounds
Models, Molecular
Phosphorus
Spectrometry, X-Ray Emission
rho GTP-Binding Proteins

Cite this

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title = "MgF(3)(-) as a transition state analog of phosphoryl transfer",

abstract = "The formation of complexes between small G proteins and certain of their effectors can be facilitated by aluminum fluorides. Solution studies suggest that magnesium may be able to replace aluminum in such complexes. We have determined the crystal structure of RhoA.GDP bound to RhoGAP in the presence of Mg(2+) and F(-) but without Al(3+). The metallofluoride adopts a trigonal planar arrangement instead of the square planar structure of AlF(4)(-). We have confirmed that these crystals contain magnesium and not aluminum by proton-induced X-ray emission spectroscopy. The structure adopted by GDP.MgF(-) possesses the stereochemistry and approximate charge expected for the transition state. We suggest that MgF3(-) may be the reagent of choice for studying phosphoryl transfer reactions.",

keywords = "Aluminum Compounds, Fluorides, Guanosine Diphosphate, Magnesium Compounds, Models, Molecular, Phosphorus, Spectrometry, X-Ray Emission, rho GTP-Binding Proteins",

author = "Graham, {Debbie L} and Lowe, {Peter N} and Grime, {Geoffrey W} and Michael Marsh and Katrin Rittinger and Smerdon, {Stephen J} and Gamblin, {Steven J} and Eccleston, {John F} and Debbie Cunningham",

year = "2002",

month = mar,

language = "English",

volume = "9",

pages = "375--81",

journal = "Chemistry & Biology",

issn = "1074-5521",

publisher = "Elsevier",

number = "3",

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TY - JOUR

T1 - MgF(3)(-) as a transition state analog of phosphoryl transfer

AU - Graham, Debbie L

AU - Lowe, Peter N

AU - Grime, Geoffrey W

AU - Marsh, Michael

AU - Rittinger, Katrin

AU - Smerdon, Stephen J

AU - Gamblin, Steven J

AU - Eccleston, John F

AU - Cunningham, Debbie

PY - 2002/3

Y1 - 2002/3

N2 - The formation of complexes between small G proteins and certain of their effectors can be facilitated by aluminum fluorides. Solution studies suggest that magnesium may be able to replace aluminum in such complexes. We have determined the crystal structure of RhoA.GDP bound to RhoGAP in the presence of Mg(2+) and F(-) but without Al(3+). The metallofluoride adopts a trigonal planar arrangement instead of the square planar structure of AlF(4)(-). We have confirmed that these crystals contain magnesium and not aluminum by proton-induced X-ray emission spectroscopy. The structure adopted by GDP.MgF(-) possesses the stereochemistry and approximate charge expected for the transition state. We suggest that MgF3(-) may be the reagent of choice for studying phosphoryl transfer reactions.

AB - The formation of complexes between small G proteins and certain of their effectors can be facilitated by aluminum fluorides. Solution studies suggest that magnesium may be able to replace aluminum in such complexes. We have determined the crystal structure of RhoA.GDP bound to RhoGAP in the presence of Mg(2+) and F(-) but without Al(3+). The metallofluoride adopts a trigonal planar arrangement instead of the square planar structure of AlF(4)(-). We have confirmed that these crystals contain magnesium and not aluminum by proton-induced X-ray emission spectroscopy. The structure adopted by GDP.MgF(-) possesses the stereochemistry and approximate charge expected for the transition state. We suggest that MgF3(-) may be the reagent of choice for studying phosphoryl transfer reactions.

KW - Aluminum Compounds

KW - Fluorides

KW - Guanosine Diphosphate

KW - Magnesium Compounds

KW - Models, Molecular

KW - Phosphorus

KW - Spectrometry, X-Ray Emission

KW - rho GTP-Binding Proteins

M3 - Article

C2 - 11927263

SN - 1074-5521

VL - 9

SP - 375

EP - 381

JO - Chemistry & Biology

JF - Chemistry & Biology

IS - 3

ER -

MgF(3)(-) as a transition state analog of phosphoryl transfer

Abstract

Keywords

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