Methods for the solubilisation of membrane proteins: the micelle-aneous world of membrane protein solubilisation

Giedre Ratkeviciute; Benjamin F. Cooper; Timothy J. Knowles

doi:10.1042/BST20210181

Methods for the solubilisation of membrane proteins: the micelle-aneous world of membrane protein solubilisation

Giedre Ratkeviciute, Benjamin F. Cooper, Timothy J. Knowles^*

^*Corresponding author for this work

Biosciences

Research output: Contribution to journal › Review article › peer-review

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Abstract

The solubilisation of membrane proteins (MPs) necessitates the overlap of two contradictory events; the extraction of MPs from their native lipid membranes and their subsequent stabilisation in aqueous environments. Whilst the current myriad of membrane mimetic systems provide a range of modus operandi, there are no golden rules for selecting the optimal pipeline for solubilisation of a specific MP hence a miscellaneous approach must be employed balancing both solubilisation efficiency and protein stability. In recent years, numerous diverse lipid membrane mimetic systems have been developed, expanding the pool of available solubilisation strategies. This review provides an overview of recent developments in the membrane mimetic field, with particular emphasis placed upon detergents, polymer-based nanodiscs and amphipols, highlighting the latest reagents to enter the toolbox of MP research.

Original language	English
Pages (from-to)	1763-1777
Number of pages	15
Journal	Biochemical Society Transactions
Volume	49
Issue number	4
DOIs	https://doi.org/10.1042/BST20210181
Publication status	Published - 20 Aug 2021

Bibliographical note

Funding Information:
We would also like to thank the funding bodies who support us. G.R. and B.F.C. are jointly funded by the BBSRC and the University of Birmingham (through the Midlands Integrative Biosciences Training Partnership — grant no. BB/M01116X/1). G.R., B.F.C. and T.J.K. are also supported by BBSRC Research grant no. BB/ S017283/1.

Keywords

membrane protein solubilisation
membrane proteins
membrane solubilisation
smalp

ASJC Scopus subject areas

Biochemistry

Access to Document

10.1042/BST20210181Licence: Creative Commons: Attribution (CC BY)

RatkeviciuteG2021MethodFinal published version, 2 MBLicence: Creative Commons: Attribution (CC BY)

Crossing the periplasmic void, elucidating the mechanisms of phospholid transport in Gram-negative bacteria
Hughes, G., Knowles, T. & Lovering, A.
Biotechnology & Biological Sciences Research Council
1/09/19 → 30/04/23
Project: Research

Cite this

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abstract = "The solubilisation of membrane proteins (MPs) necessitates the overlap of two contradictory events; the extraction of MPs from their native lipid membranes and their subsequent stabilisation in aqueous environments. Whilst the current myriad of membrane mimetic systems provide a range of modus operandi, there are no golden rules for selecting the optimal pipeline for solubilisation of a specific MP hence a miscellaneous approach must be employed balancing both solubilisation efficiency and protein stability. In recent years, numerous diverse lipid membrane mimetic systems have been developed, expanding the pool of available solubilisation strategies. This review provides an overview of recent developments in the membrane mimetic field, with particular emphasis placed upon detergents, polymer-based nanodiscs and amphipols, highlighting the latest reagents to enter the toolbox of MP research.",

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Methods for the solubilisation of membrane proteins: the micelle-aneous world of membrane protein solubilisation

Abstract

Bibliographical note

Keywords

ASJC Scopus subject areas

Access to Document

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Projects

Crossing the periplasmic void, elucidating the mechanisms of phospholid transport in Gram-negative bacteria

Cite this