Methods for Structure Determination of SH2 Domain–Phosphopeptide Complexes by NMR

Vittoria Nanna; Michelangelo Marasco; John Kirkpatrick; Teresa Carlomagno

doi:10.1007/978-1-0716-3393-9_1

Methods for Structure Determination of SH2 Domain–Phosphopeptide Complexes by NMR

Vittoria Nanna, Michelangelo Marasco, John Kirkpatrick, Teresa Carlomagno^*

^*Corresponding author for this work

Biosciences

Research output: Chapter in Book/Report/Conference proceeding › Chapter (peer-reviewed) › peer-review

Abstract

Nuclear magnetic resonance (NMR) spectroscopy is a powerful technique to solve the structure of biomolecular complexes at atomic resolution in solution. Small proteins such as Src-homology 2 (SH2) domains have fast tumbling rates and long-lived NMR signals, making them particularly suited to be studied by standard NMR methods. SH2 domains are modular proteins whose function is the recognition of sequences containing phosphotyrosines. In this chapter, we describe the application of NMR to assess the interaction between SH2 domains and phosphopeptides and determine the structure of the resulting complexes.

Original language	English
Title of host publication	SH2 Domains
Subtitle of host publication	Functional Modules and Evolving Tools in Biology
Editors	Teresa Carlomagno, Maja Köhn
Publisher	Humana Press
Pages	3-23
Number of pages	20
ISBN (Electronic)	9781071633939
ISBN (Print)	9781071633922, 9781071633953
DOIs	https://doi.org/10.1007/978-1-0716-3393-9_1
Publication status	Published - 6 Sept 2023

Publication series

Name	Methods in molecular biology
Publisher	Springer
Volume	2705
ISSN (Print)	1064-3745
ISSN (Electronic)	1940-6029

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Methods for Structure Determination of SH2 Domain–Phosphopeptide Complexes by NMR. / Nanna, Vittoria; Marasco, Michelangelo; Kirkpatrick, John et al.
SH2 Domains: Functional Modules and Evolving Tools in Biology. ed. / Teresa Carlomagno; Maja Köhn. Humana Press, 2023. p. 3-23 (Methods in molecular biology; Vol. 2705).

Research output: Chapter in Book/Report/Conference proceeding › Chapter (peer-reviewed) › peer-review

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AU - Marasco, Michelangelo

AU - Kirkpatrick, John

AU - Carlomagno, Teresa

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DO - 10.1007/978-1-0716-3393-9_1

M3 - Chapter (peer-reviewed)

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A2 - Carlomagno, Teresa

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ER -

Methods for Structure Determination of SH2 Domain–Phosphopeptide Complexes by NMR

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