Metabonomics of human fecal extracts characterize ulcerative colitis, Crohn’s disease and healthy individuals

Jacob Tveiten Bjerrum, Yulan Wang*, Fuhua Hao, Mehmet Coskun, Christian Ludwig, Ulrich Günther, Ole Haagen Nielsen

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

120 Citations (Scopus)
32 Downloads (Pure)

Abstract

This study employs spectroscopy-based metabolic profiling of fecal extracts from healthy subjects and patients with active or inactive ulcerative colitis (UC) and Crohn’s disease (CD) to substantiate the potential use of spectroscopy as a non-invasive diagnostic tool and to characterize the fecal metabolome in inflammatory bowel disease (IBD). Stool samples from 113 individuals (UC 48, CD 44, controls 21) were analyzed by 1H nuclear magnetic resonance (NMR) spectroscopy (Bruker 600 MHz, Bruker BioSpin, Rheinstetten, Germany). Data were analyzed with principal component analysis and orthogonal-projection to latent structure-discriminant analysis using SIMCA-P + 12 and MATLAB. Significant differences were found in the metabolic profiles making it possible to differentiate between active IBD and controls and between UC and CD. The metabolites holding differential power primarily belonged to a range of amino acids, microbiota-related short chain fatty acids, and lactate suggestive of an inflammation-driven malabsorption and dysbiosis of the normal bacterial ecology. However, removal of patients with intestinal surgery and anti-TNF-α antibody treatment eliminated the discriminative power regarding UC versus CD. This study consequently demonstrates that 1H NMR spectroscopy of fecal extracts is a potential non-invasive diagnostic tool and able to characterize the inflammation-driven changes in the metabolic profiles related to malabsorption and dysbiosis. Intestinal surgery and medication are to be accounted for in future studies, as it seems to be factors of importance in the discriminative process.

Original languageEnglish
Pages (from-to)122-133
Number of pages12
JournalMetabolomics
Volume11
Issue number1
Early online date1 Jun 2014
DOIs
Publication statusPublished - Feb 2015

Keywords

  • Diagnostic tool
  • Dysbiosis
  • Inflammatory bowel disease
  • Metabolomics
  • NMR spectroscopy

ASJC Scopus subject areas

  • Endocrinology, Diabetes and Metabolism
  • Biochemistry
  • Clinical Biochemistry

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