Metabolic footprinting as a tool for discriminating between brewing yeasts

Georgina A Pope; Donald A MacKenzie; Marianne Defernez; Miguel A M M Aroso; Linda J Fuller; Fred A Mellon; Warwick B Dunn; Marie Brown; Royston Goodacre; Douglas B Kell; Marcus E Marvin; Edward J Louis; Ian N Roberts

doi:10.1002/yea.1499

Metabolic footprinting as a tool for discriminating between brewing yeasts

Georgina A Pope, Donald A MacKenzie, Marianne Defernez, Miguel A M M Aroso, Linda J Fuller, Fred A Mellon, Warwick B Dunn, Marie Brown, Royston Goodacre, Douglas B Kell, Marcus E Marvin, Edward J Louis, Ian N Roberts

Biosciences

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84 Citations (Scopus)

Abstract

The characterization of industrial yeast strains by examining their metabolic footprints (exometabolomes) was investigated and compared to genome-based discriminatory methods. A group of nine industrial brewing yeasts was studied by comparing their metabolic footprints, genetic fingerprints and comparative genomic hybridization profiles. Metabolic footprinting was carried out by both direct injection mass spectrometry (DIMS) and gas chromatography time-of-flight mass spectrometry (GC-TOF-MS), with data analysed by principal components analysis (PCA) and canonical variates analysis (CVA). The genomic profiles of the nine yeasts were compared by PCR-restriction fragment length polymorphism (PCR-RFLP) analysis, genetic fingerprinting using amplified fragment length polymorphism (AFLP) analysis and microarray comparative genome hybridizations (CGH). Metabolomic and genomic analysis comparison of the nine brewing yeasts identified metabolomics as a powerful tool in separating genotypically and phenotypically similar strains. For some strains discrimination not achieved genomically was observed metabolomically.

Original language	English
Pages (from-to)	667-79
Number of pages	13
Journal	Yeast
Volume	24
Issue number	8
DOIs	https://doi.org/10.1002/yea.1499
Publication status	Published - Aug 2007

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@article{792bd6b6103744cda0350a40f683e7d2,

title = "Metabolic footprinting as a tool for discriminating between brewing yeasts",

abstract = "The characterization of industrial yeast strains by examining their metabolic footprints (exometabolomes) was investigated and compared to genome-based discriminatory methods. A group of nine industrial brewing yeasts was studied by comparing their metabolic footprints, genetic fingerprints and comparative genomic hybridization profiles. Metabolic footprinting was carried out by both direct injection mass spectrometry (DIMS) and gas chromatography time-of-flight mass spectrometry (GC-TOF-MS), with data analysed by principal components analysis (PCA) and canonical variates analysis (CVA). The genomic profiles of the nine yeasts were compared by PCR-restriction fragment length polymorphism (PCR-RFLP) analysis, genetic fingerprinting using amplified fragment length polymorphism (AFLP) analysis and microarray comparative genome hybridizations (CGH). Metabolomic and genomic analysis comparison of the nine brewing yeasts identified metabolomics as a powerful tool in separating genotypically and phenotypically similar strains. For some strains discrimination not achieved genomically was observed metabolomically.",

author = "Pope, {Georgina A} and MacKenzie, {Donald A} and Marianne Defernez and Aroso, {Miguel A M M} and Fuller, {Linda J} and Mellon, {Fred A} and Dunn, {Warwick B} and Marie Brown and Royston Goodacre and Kell, {Douglas B} and Marvin, {Marcus E} and Louis, {Edward J} and Roberts, {Ian N}",

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language = "English",

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pages = "667--79",

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T1 - Metabolic footprinting as a tool for discriminating between brewing yeasts

AU - Pope, Georgina A

AU - MacKenzie, Donald A

AU - Defernez, Marianne

AU - Aroso, Miguel A M M

AU - Fuller, Linda J

AU - Mellon, Fred A

AU - Dunn, Warwick B

AU - Brown, Marie

AU - Goodacre, Royston

AU - Kell, Douglas B

AU - Marvin, Marcus E

AU - Louis, Edward J

AU - Roberts, Ian N

PY - 2007/8

Y1 - 2007/8

N2 - The characterization of industrial yeast strains by examining their metabolic footprints (exometabolomes) was investigated and compared to genome-based discriminatory methods. A group of nine industrial brewing yeasts was studied by comparing their metabolic footprints, genetic fingerprints and comparative genomic hybridization profiles. Metabolic footprinting was carried out by both direct injection mass spectrometry (DIMS) and gas chromatography time-of-flight mass spectrometry (GC-TOF-MS), with data analysed by principal components analysis (PCA) and canonical variates analysis (CVA). The genomic profiles of the nine yeasts were compared by PCR-restriction fragment length polymorphism (PCR-RFLP) analysis, genetic fingerprinting using amplified fragment length polymorphism (AFLP) analysis and microarray comparative genome hybridizations (CGH). Metabolomic and genomic analysis comparison of the nine brewing yeasts identified metabolomics as a powerful tool in separating genotypically and phenotypically similar strains. For some strains discrimination not achieved genomically was observed metabolomically.

AB - The characterization of industrial yeast strains by examining their metabolic footprints (exometabolomes) was investigated and compared to genome-based discriminatory methods. A group of nine industrial brewing yeasts was studied by comparing their metabolic footprints, genetic fingerprints and comparative genomic hybridization profiles. Metabolic footprinting was carried out by both direct injection mass spectrometry (DIMS) and gas chromatography time-of-flight mass spectrometry (GC-TOF-MS), with data analysed by principal components analysis (PCA) and canonical variates analysis (CVA). The genomic profiles of the nine yeasts were compared by PCR-restriction fragment length polymorphism (PCR-RFLP) analysis, genetic fingerprinting using amplified fragment length polymorphism (AFLP) analysis and microarray comparative genome hybridizations (CGH). Metabolomic and genomic analysis comparison of the nine brewing yeasts identified metabolomics as a powerful tool in separating genotypically and phenotypically similar strains. For some strains discrimination not achieved genomically was observed metabolomically.

U2 - 10.1002/yea.1499

DO - 10.1002/yea.1499

M3 - Article

C2 - 17534862

SN - 0749-503X

VL - 24

SP - 667

EP - 679

JO - Yeast

JF - Yeast

IS - 8

ER -

Metabolic footprinting as a tool for discriminating between brewing yeasts

Abstract

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