Membrane blebbing during apoptosis results from caspase-mediated activation of ROCK I

M L Coleman; E A Sahai; M Yeo; M Bosch; A Dewar; M F Olson

doi:10.1038/35070009

Membrane blebbing during apoptosis results from caspase-mediated activation of ROCK I

M L Coleman, E A Sahai, M Yeo, M Bosch, A Dewar, M F Olson

Cancer and Genomic Sciences

Research output: Contribution to journal › Article › peer-review

900 Citations (Scopus)

Abstract

The execution phase of apoptosis is characterized by marked changes in cell morphology that include contraction and membrane blebbing. The actin-myosin system has been proposed to be the source of contractile force that drives bleb formation, although the biochemical pathway that promotes actin-myosin contractility during apoptosis has not been identified. Here we show that the Rho effector protein ROCK I, which contributes to phosphorylation of myosin light-chains, myosin ATPase activity and coupling of actin-myosin filaments to the plasma membrane, is cleaved during apoptosis to generate a truncated active form. The activity of ROCK proteins is both necessary and sufficient for formation of membrane blebs and for re-localization of fragmented DNA into blebs and apoptotic bodies.

Original language	English
Pages (from-to)	339-45
Number of pages	7
Journal	Nature Cell Biology
Volume	3
Issue number	4
DOIs	https://doi.org/10.1038/35070009
Publication status	Published - Apr 2001

Keywords

3T3 Cells
Animals
Apoptosis
Caspase Inhibitors
Caspases
Cell Membrane
DNA
DNA Fragmentation
Enzyme Activation
Humans
Intracellular Signaling Peptides and Proteins
Mice
Protein-Serine-Threonine Kinases
Recombinant Fusion Proteins
Tumor Cells, Cultured
rho-Associated Kinases

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10.1038/35070009

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title = "Membrane blebbing during apoptosis results from caspase-mediated activation of ROCK I",

abstract = "The execution phase of apoptosis is characterized by marked changes in cell morphology that include contraction and membrane blebbing. The actin-myosin system has been proposed to be the source of contractile force that drives bleb formation, although the biochemical pathway that promotes actin-myosin contractility during apoptosis has not been identified. Here we show that the Rho effector protein ROCK I, which contributes to phosphorylation of myosin light-chains, myosin ATPase activity and coupling of actin-myosin filaments to the plasma membrane, is cleaved during apoptosis to generate a truncated active form. The activity of ROCK proteins is both necessary and sufficient for formation of membrane blebs and for re-localization of fragmented DNA into blebs and apoptotic bodies.",

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author = "Coleman, {M L} and Sahai, {E A} and M Yeo and M Bosch and A Dewar and Olson, {M F}",

year = "2001",

month = apr,

doi = "10.1038/35070009",

language = "English",

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pages = "339--45",

journal = "Nature Cell Biology",

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T1 - Membrane blebbing during apoptosis results from caspase-mediated activation of ROCK I

AU - Coleman, M L

AU - Sahai, E A

AU - Yeo, M

AU - Bosch, M

AU - Dewar, A

AU - Olson, M F

PY - 2001/4

Y1 - 2001/4

N2 - The execution phase of apoptosis is characterized by marked changes in cell morphology that include contraction and membrane blebbing. The actin-myosin system has been proposed to be the source of contractile force that drives bleb formation, although the biochemical pathway that promotes actin-myosin contractility during apoptosis has not been identified. Here we show that the Rho effector protein ROCK I, which contributes to phosphorylation of myosin light-chains, myosin ATPase activity and coupling of actin-myosin filaments to the plasma membrane, is cleaved during apoptosis to generate a truncated active form. The activity of ROCK proteins is both necessary and sufficient for formation of membrane blebs and for re-localization of fragmented DNA into blebs and apoptotic bodies.

AB - The execution phase of apoptosis is characterized by marked changes in cell morphology that include contraction and membrane blebbing. The actin-myosin system has been proposed to be the source of contractile force that drives bleb formation, although the biochemical pathway that promotes actin-myosin contractility during apoptosis has not been identified. Here we show that the Rho effector protein ROCK I, which contributes to phosphorylation of myosin light-chains, myosin ATPase activity and coupling of actin-myosin filaments to the plasma membrane, is cleaved during apoptosis to generate a truncated active form. The activity of ROCK proteins is both necessary and sufficient for formation of membrane blebs and for re-localization of fragmented DNA into blebs and apoptotic bodies.

KW - 3T3 Cells

KW - Animals

KW - Apoptosis

KW - Caspase Inhibitors

KW - Caspases

KW - Cell Membrane

KW - DNA

KW - DNA Fragmentation

KW - Enzyme Activation

KW - Humans

KW - Intracellular Signaling Peptides and Proteins

KW - Mice

KW - Protein-Serine-Threonine Kinases

KW - Recombinant Fusion Proteins

KW - Tumor Cells, Cultured

KW - rho-Associated Kinases

U2 - 10.1038/35070009

DO - 10.1038/35070009

M3 - Article

C2 - 11283606

SN - 1465-7392

VL - 3

SP - 339

EP - 345

JO - Nature Cell Biology

JF - Nature Cell Biology

IS - 4

ER -

Membrane blebbing during apoptosis results from caspase-mediated activation of ROCK I

Abstract

Keywords

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