Measuring IP3 Generation in Real-Time Using a NanoBiT Luminescence Biosensor

Caroline M. Gorvin

doi:10.1007/978-1-0716-4164-4_3

Measuring IP3 Generation in Real-Time Using a NanoBiT Luminescence Biosensor

Caroline M. Gorvin

Metabolism and Systems Science

Research output: Chapter in Book/Report/Conference proceeding › Chapter (peer-reviewed) › peer-review

Abstract

G protein-coupled receptors that activate Gq/11 regulate a range of physiological processes including neurotransmission, energy homeostasis, blood pressure regulation, and calcium homeostasis. Activation of Gq/11-coupled receptors stimulates the generation of inositol 1,4,5-trisphosphate (IP3), which mobilizes intracellular calcium release from the endoplasmic reticulum. This chapter describes an assay that uses a NanoBiT-IP3 luminescent biosensor to detect increases in IP3 in live cells. It describes how to perform these assays to assess signaling by the ghrelin receptor and the calcium-sensing receptor in HEK293 cells.

Original language	English
Title of host publication	Calcium Signaling
Subtitle of host publication	Methods and Protocols
Editors	Caroline M. Gorvin
Place of Publication	New York, NY
Publisher	Humana Press
Pages	33-42
Number of pages	10
Edition	1
ISBN (Electronic)	9781071641644
ISBN (Print)	9781071641637, 9781071641668
DOIs	https://doi.org/10.1007/978-1-0716-4164-4_3
Publication status	Published - 13 Oct 2024

Publication series

Name	Methods in molecular biology
Publisher	Springer
Volume	2861
ISSN (Print)	1064-3745
ISSN (Electronic)	1940-6029

Bibliographical note

Publisher Copyright:
© 2025. The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature.

Keywords

Calcium-sensing receptor
Ghrelin receptor
Gq/11 signaling
Luminescence
Phospholipase C

ASJC Scopus subject areas

Molecular Biology
Genetics

Access to Document

10.1007/978-1-0716-4164-4_3

Deciphering the complexities in ghrelin receptor signalling to improve understanding of appetite regulation
Gorvin, C. (Principal Investigator)
The Wellcome Trust
1/08/22 → 31/07/27
Project: Research

Cite this

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Measuring IP3 Generation in Real-Time Using a NanoBiT Luminescence Biosensor

Abstract

Publication series

Bibliographical note

Keywords

ASJC Scopus subject areas

Access to Document

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Projects

Deciphering the complexities in ghrelin receptor signalling to improve understanding of appetite regulation

Cite this