Measurement of Drosophila Reproductive Behaviors

Anne C von Philipsborn, Galit Shohat-Ophir*, Carolina Rezaval*

*Corresponding author for this work

Research output: Contribution to journalArticle


Courtship behaviors in Drosophila melanogaster are innate and contain highly stereotyped but also experience- and state-dependent elements. They have been the subject of intense study for more than 100 years. The power of Drosophila as a genetic experimental system has allowed the dissection of reproductive behaviors at a molecular, cellular, and physiological level. As a result, we know a great deal about how flies perceive sensory cues from potential mates, how this information is integrated in higher brain centers to execute reproductive decisions, and how state and social contexts modulate these responses. The simplicity of the assay has allowed for its broad application. Here, we introduce methods for studying male and female innate reproductive behaviors as well as their plastic responses.

Original languageEnglish
Pages (from-to)431-439
Number of pages9
JournalCold Spring Harbor Protocols
Issue number7
Early online date13 Feb 2023
Publication statusPublished - Jul 2023

Bibliographical note

© 2023 Cold Spring Harbor Laboratory Press.


  • Animals
  • Male
  • Female
  • Drosophila/genetics
  • Drosophila melanogaster/genetics
  • Sexual Behavior, Animal/physiology
  • Courtship
  • Drosophila Proteins/genetics


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