TY - JOUR
T1 - Measurement of chromosomal DNA single-strand breaks and replication fork progression rates
AU - Breslin, Claire
AU - Clements, Paula M
AU - El-Khamisy, Sherif F
AU - Petermann, Eva
AU - Iles, Natasha
AU - Caldecott, Keith W
PY - 2006
Y1 - 2006
N2 - Chromosomal single-strand breaks (SSBs) are the most common lesions arising in cells, but are normally rapidly repaired by multiprotein complexes centered around the scaffold protein, XRCC1. Here, we describe protocols to measure chromosomal SSBs in cells and for recovering and identifying novel components of SSBR complexes in vitro and in vivo. We also describe an assay we employ to measure the rate of replication fork progression in mammalian/vertebrate cells in the presence or absence of DNA damage.
AB - Chromosomal single-strand breaks (SSBs) are the most common lesions arising in cells, but are normally rapidly repaired by multiprotein complexes centered around the scaffold protein, XRCC1. Here, we describe protocols to measure chromosomal SSBs in cells and for recovering and identifying novel components of SSBR complexes in vitro and in vivo. We also describe an assay we employ to measure the rate of replication fork progression in mammalian/vertebrate cells in the presence or absence of DNA damage.
U2 - 10.1016/S0076-6879(05)09024-5
DO - 10.1016/S0076-6879(05)09024-5
M3 - Review article
C2 - 16793415
SN - 0076-6879
VL - 409
SP - 410
EP - 425
JO - Methods in Enzymology
JF - Methods in Enzymology
ER -