Projects per year
Abstract
Here, we demonstrate detection by mass spectrometry of an intact protein-drug complex directly from liver tissue from rats that had been orally dosed with the drug. The protein-drug complex comprised fatty acid binding protein 1, FABP1, non-covalently bound to the small molecule therapeutic bezafibrate. Moreover, we demonstrate spatial mapping of the [FABP1+bezafibrate] complex across a thin section of liver by targeted mass spectrometry imaging. This work is the first demonstration of in situ mass spectrometry analysis of a non-covalent protein-drug complex formed in vivo and has implications for early stage drug discovery by providing a route to target-drug characterization directly from the physiological environment.
Original language | English |
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Article number | e202202075 |
Journal | Angewandte Chemie (International Edition) |
Volume | 61 |
Issue number | 36 |
Early online date | 13 Jul 2022 |
DOIs | |
Publication status | E-pub ahead of print - 13 Jul 2022 |
Bibliographical note
© 2022 The Authors. Angewandte Chemie International Edition published by Wiley-VCH GmbH.Keywords
- Ex vivo Tissue
- Mass Spectrometry Imaging
- Native Ambient Mass Spectrometry
- Protein–Drug Complex
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Dive into the research topics of 'Mass Spectrometry Detection and Imaging of a Non-Covalent Protein-Drug Complex in Tissue from Orally Dosed Rats'. Together they form a unique fingerprint.Projects
- 3 Finished
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A new mass spectrometer for structural proteomics and protein imaging
Thomas, C., Grant, M., Lovering, A., Alderwick, L., Tomlinson, M., Winn, P., Knowles, T., Cooper, H., Styles, I., Gibbs, D., Huber, D., Bhatt, A. & Leney, A.
Biotechnology & Biological Sciences Research Council
1/07/19 → 30/06/20
Project: Research
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NAMS: Native ambient mass spectrometry
Engineering & Physical Science Research Council
1/06/19 → 30/11/23
Project: Research
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A new tool to support drug discovery: Native LESA mass spectrometry (NESA)
Engineering & Physical Science Research Council
1/04/18 → 31/03/21
Project: Research Councils
Datasets
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Data to accompany "Mass Spectrometry Detection and Imaging of a Non-Covalent Protein-Drug Complex in Tissue from Orally-Dosed Rats"
Cooper, H. (Creator), University of Birmingham, 30 May 2022
DOI: https://doi.org/10.25500/edata.bham.00000845
Dataset