Manufacturing of agarose-based chromatographic adsorbents with controlled pore and particle sizes

Nicolas Ioannidis; Andrzej W. Pacek; James Bowen; Zhibing Zhang

Manufacturing of agarose-based chromatographic adsorbents with controlled pore and particle sizes

Nicolas Ioannidis^*, Andrzej W. Pacek, James Bowen, Zhibing Zhang

^*Corresponding author for this work

Chemical Engineering

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution

Abstract

The effect of ionic strength and quenching temperature on the mechanical properties and structure of agarose-based chromatographic adsorbents in micro-beads for bioseparation was investigated. The pore size/size distribution of the beads was measured by analysis of their AFM images. Their mechanical properties were determined by a micromanipulation technique based on compression of single micro-particles. It was found that the mean pore size and stiffness of agarose beads increase with ionic strength and slow cooling. These two parameters do not affect the particle size/size distribution.

Original language	English
Title of host publication	Annual Technical Conference - ANTEC, Conference Proceedings
Pages	1025-1031
Number of pages	7
Volume	2
Publication status	Published - 2012
Event	69th Annual Technical Conference of the Society of Plastics Engineers 2011, ANTEC 2011 - Boston, MA, United States Duration: 1 May 2011 → 5 May 2011

Conference

Conference	69th Annual Technical Conference of the Society of Plastics Engineers 2011, ANTEC 2011
Country/Territory	United States
City	Boston, MA
Period	1/05/11 → 5/05/11

ASJC Scopus subject areas

Polymers and Plastics
General Chemical Engineering

Cite this

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title = "Manufacturing of agarose-based chromatographic adsorbents with controlled pore and particle sizes",

abstract = "The effect of ionic strength and quenching temperature on the mechanical properties and structure of agarose-based chromatographic adsorbents in micro-beads for bioseparation was investigated. The pore size/size distribution of the beads was measured by analysis of their AFM images. Their mechanical properties were determined by a micromanipulation technique based on compression of single micro-particles. It was found that the mean pore size and stiffness of agarose beads increase with ionic strength and slow cooling. These two parameters do not affect the particle size/size distribution.",

author = "Nicolas Ioannidis and Pacek, {Andrzej W.} and James Bowen and Zhibing Zhang",

year = "2012",

language = "English",

isbn = "9781617829604",

volume = "2",

pages = "1025--1031",

booktitle = "Annual Technical Conference - ANTEC, Conference Proceedings",

note = "69th Annual Technical Conference of the Society of Plastics Engineers 2011, ANTEC 2011 ; Conference date: 01-05-2011 Through 05-05-2011",

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TY - GEN

T1 - Manufacturing of agarose-based chromatographic adsorbents with controlled pore and particle sizes

AU - Ioannidis, Nicolas

AU - Pacek, Andrzej W.

AU - Bowen, James

AU - Zhang, Zhibing

PY - 2012

Y1 - 2012

N2 - The effect of ionic strength and quenching temperature on the mechanical properties and structure of agarose-based chromatographic adsorbents in micro-beads for bioseparation was investigated. The pore size/size distribution of the beads was measured by analysis of their AFM images. Their mechanical properties were determined by a micromanipulation technique based on compression of single micro-particles. It was found that the mean pore size and stiffness of agarose beads increase with ionic strength and slow cooling. These two parameters do not affect the particle size/size distribution.

AB - The effect of ionic strength and quenching temperature on the mechanical properties and structure of agarose-based chromatographic adsorbents in micro-beads for bioseparation was investigated. The pore size/size distribution of the beads was measured by analysis of their AFM images. Their mechanical properties were determined by a micromanipulation technique based on compression of single micro-particles. It was found that the mean pore size and stiffness of agarose beads increase with ionic strength and slow cooling. These two parameters do not affect the particle size/size distribution.

UR - http://www.scopus.com/inward/record.url?scp=80051817326&partnerID=8YFLogxK

M3 - Conference contribution

AN - SCOPUS:80051817326

SN - 9781617829604

VL - 2

SP - 1025

EP - 1031

BT - Annual Technical Conference - ANTEC, Conference Proceedings

T2 - 69th Annual Technical Conference of the Society of Plastics Engineers 2011, ANTEC 2011

Y2 - 1 May 2011 through 5 May 2011

ER -

Manufacturing of agarose-based chromatographic adsorbents with controlled pore and particle sizes

Abstract

Conference

ASJC Scopus subject areas

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