Projects per year
Abstract
Transcription of the 74 kb Pseudomonas fluorescens mupirocin [pseudomonic acid (PA)] biosynthesis cluster depends on quorum sensing-dependent regulation via the LuxI/LuxR homologues MupI/MupR. To facilitate analysis of novel PAs from pathway mutants, we investigated factors that affect mup gene expression. First, the signal produced by MupI was identified as N-(3-oxodecanoyl)homoserine lactone, but exogenous addition of this molecule did not activate mupirocin production prematurely nor did expression of mupI in trans increase metabolite production. Second, we confirmed that mupX, encoding an amidase/hydrolase that can degrade N-acylhomoserine lactones, is also required for efficient expression, consistent with its occurrence in a regulatory module linked to unrelated genes in P. fluorescens. Third, and most significantly, mupR expression in trans to wild type and mutants can increase production of antibiotic and novel intermediates up to 17-fold.
Original language | English |
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Journal | Applied Microbiology and Biotechnology |
DOIs | |
Publication status | Published - 12 Feb 2011 |
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Dive into the research topics of 'Manipulation of quorum sensing regulation in Pseudomonas fluorescens NCIMB 10586 to increase mupirocin production.'. Together they form a unique fingerprint.Projects
- 2 Finished
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Biosynthesis of polyketide antibiotic mupirocin by Pseudomonas fluorescens
Biotechnology & Biological Sciences Research Council
1/06/07 → 30/01/11
Project: Research Councils
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Biosynthesis of the Polyketide Antibiotic Mupirocin by Pseudomonas Fluorescens
Biotechnology & Biological Sciences Research Council
14/05/04 → 13/05/07
Project: Research Councils