MALDI imaging of liquid extraction surface analysis sampled tissue

Elizabeth Randall, Alan Race, Helen Cooper, Josephine Bunch

Research output: Contribution to journalArticlepeer-review

17 Citations (Scopus)
162 Downloads (Pure)


Combined mass spectrometry imaging methods in which two different techniques are executed on the same sample have recently been reported for a number of sample types. Such an approach can be used to examine the sampling effects of the first technique with a second, higher resolution method and also combines the advantages of each technique for a more complete analysis. In this work MALDI MSI was used to study the effects of LESA sampling on mouse brain tissue. Complementary multivariate analysis techniques including principal component analysis, non-negative matrix factorization and t-distributed stochastic neighbour embedding, were applied to MALDI MS images acquired from tissue which had been sampled by LESA to gain a better understanding of localized tissue washing in LESA sampling. It was found that MALDI MS images could be used to visualize regions sampled by LESA. The variability in sampling area, spatial precision and delocalization of analytes in tissue induced by LESA were assessed using both single ion images and images provided by multivariate analysis.
Original languageEnglish
Pages (from-to)8433–8440
JournalAnalytical Chemistry
Issue number17
Early online date22 Jul 2016
Publication statusPublished - 6 Sept 2016


  • Liquid extraction surface analysis
  • multimodal mass spectrometry imaging
  • data registration
  • metrology


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