Light-controlled thrombin catalysis and clot formation using a photoswitchable G-quadruplex DNA aptamer

Aysha Ali; Gemma A Bullen; Benjamin Cross; Timothy R Dafforn; Haydn A Little; Jack Manchester; Anna F A Peacock; James H R Tucker

doi:10.1039/c9cc01540j

Light-controlled thrombin catalysis and clot formation using a photoswitchable G-quadruplex DNA aptamer

Aysha Ali, Gemma A Bullen, Benjamin Cross, Timothy R Dafforn, Haydn A Little, Jack Manchester, Anna F A Peacock, James H R Tucker

Research output: Contribution to journal › Article › peer-review

5 Citations (Scopus)

238 Downloads (Pure)

Abstract

The reversible photocontrol of an enzyme governing blood coagulation is demonstrated. The thrombin binding aptamer (TBA), was rendered photochromic by modification with two anthracene groups. Light-triggered anthracene photodimerisation distorts its structure, inhibiting binding of the enzyme thrombin, which in turn triggers catalysis and the resulting clotting process.

Original language	English
Pages (from-to)	5627-5630
Number of pages	4
Journal	Chemical Communications
Volume	55
Issue number	39
Early online date	16 Apr 2019
DOIs	https://doi.org/10.1039/c9cc01540j
Publication status	Published - 16 Apr 2019

ASJC Scopus subject areas

Catalysis
Electronic, Optical and Magnetic Materials
Ceramics and Composites
General Chemistry
Surfaces, Coatings and Films
Metals and Alloys
Materials Chemistry

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10.1039/c9cc01540jLicence: Creative Commons: Attribution (CC BY)

Ali_et_al_Light_controlled_thrombin_catalysis_Chemical_Communications_2019
Checked for eligibility: 30/04/2019 Tucker, J. H. R., et al. "Light-controlled Thrombin Catalysis and Clot Formation using a Photoswitchable G-Quadruplex DNA Aptamer." Chemical Communications (2019).
Final published version, 2.08 MBLicence: Creative Commons: Attribution (CC BY)

Functional DNA-based assemblies
Tucker, J.
Engineering & Physical Science Research Council
16/03/09 → 31/03/14
Project: Research Councils

Cite this

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AU - Ali, Aysha

AU - Bullen, Gemma A

AU - Cross, Benjamin

AU - Dafforn, Timothy R

AU - Little, Haydn A

AU - Manchester, Jack

AU - Peacock, Anna F A

AU - Tucker, James H R

PY - 2019/4/16

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AB - The reversible photocontrol of an enzyme governing blood coagulation is demonstrated. The thrombin binding aptamer (TBA), was rendered photochromic by modification with two anthracene groups. Light-triggered anthracene photodimerisation distorts its structure, inhibiting binding of the enzyme thrombin, which in turn triggers catalysis and the resulting clotting process.

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Light-controlled thrombin catalysis and clot formation using a photoswitchable G-quadruplex DNA aptamer

Abstract

ASJC Scopus subject areas

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Functional DNA-based assemblies

Cite this