Light-controlled thrombin catalysis and clot formation using a photoswitchable G-quadruplex DNA aptamer

Aysha Ali, Gemma A Bullen, Benjamin Cross, Timothy R Dafforn, Haydn A Little, Jack Manchester, Anna F A Peacock, James H R Tucker

Research output: Contribution to journalArticlepeer-review

5 Citations (Scopus)
163 Downloads (Pure)

Abstract

The reversible photocontrol of an enzyme governing blood coagulation is demonstrated. The thrombin binding aptamer (TBA), was rendered photochromic by modification with two anthracene groups. Light-triggered anthracene photodimerisation distorts its structure, inhibiting binding of the enzyme thrombin, which in turn triggers catalysis and the resulting clotting process.

Original languageEnglish
Pages (from-to)5627-5630
Number of pages4
JournalChemical Communications
Volume55
Issue number39
Early online date16 Apr 2019
DOIs
Publication statusPublished - 16 Apr 2019

ASJC Scopus subject areas

  • Catalysis
  • Electronic, Optical and Magnetic Materials
  • Ceramics and Composites
  • Chemistry(all)
  • Surfaces, Coatings and Films
  • Metals and Alloys
  • Materials Chemistry

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