Leukocyte heat shock protein expression before and after intensified training

Martin Whitham, Shona Halson, Graeme Lancaster, M Gleeson, Asker Jeukendrup, Andrew Blannin

Research output: Contribution to journalArticlepeer-review

12 Citations (Scopus)


The purpose of the current research was to test the hypothesis that exercise induced leukocyte heat shock protein (HSP) expression is increased during periods of intensified exercise training. Seven male endurance cyclists carried out tests of maximal oxygen consumption and endurance capacity. These standard exercise tests were carried out prior to and following 6 days of prescribed intensified training. Sampled leukocytes were examined for Hsp27 and Hsp70 expression using a Fluorescence Activated Cell Scanner (EPICS XL, Coulter). During a period of overreaching, as signified by a drop in time to fatigue following the intensified training period (p = 0.02), the number of extracellular Hsp27 positive granulocytes increased in response to the VO(2)max test. Acute, intracellular HSP responses were observed in both baseline and overreached conditions. The present study showed that a period of intensified training caused adaptations in the acute heat shock protein exercise response, reflected by a greater increase of cell surface HSP positive leukocytes following heavy training.

Original languageEnglish
Pages (from-to)522-527
Number of pages6
JournalInternational Journal of Sports Medicine
Issue number7
Publication statusPublished - 1 Jan 2004


  • Adaptation, Physiological
  • Adult
  • Bicycling
  • Exercise
  • Heat-Shock Proteins
  • Humans
  • Leukocytes
  • Male
  • Reference Values
  • Journal Article


Dive into the research topics of 'Leukocyte heat shock protein expression before and after intensified training'. Together they form a unique fingerprint.

Cite this