TY - JOUR
T1 - Kinesin-8 motors improve nuclear centering by promoting microtubule catastrophe
AU - Glunčić, Matko
AU - Maghelli, Nicola
AU - Krull, Alexander
AU - Krstić, Vladimir
AU - Ramunno-Johnson, Damien
AU - Pavin, Nenad
AU - Tolić, Iva M
PY - 2015/2/18
Y1 - 2015/2/18
N2 - In fission yeast, microtubules push against the cell edge, thereby positioning the nucleus in the cell center. Kinesin-8 motors regulate microtubule catastrophe; however, their role in nuclear positioning is not known. Here we develop a physical model that describes how kinesin-8 motors affect nuclear centering by promoting a microtubule catastrophe. Our model predicts the improved centering of the nucleus in the presence of motors, which we confirmed experimentally in living cells. The model also predicts a characteristic time for the recentering of a displaced nucleus, which is supported by our experiments where we displaced the nucleus using optical tweezers
AB - In fission yeast, microtubules push against the cell edge, thereby positioning the nucleus in the cell center. Kinesin-8 motors regulate microtubule catastrophe; however, their role in nuclear positioning is not known. Here we develop a physical model that describes how kinesin-8 motors affect nuclear centering by promoting a microtubule catastrophe. Our model predicts the improved centering of the nucleus in the presence of motors, which we confirmed experimentally in living cells. The model also predicts a characteristic time for the recentering of a displaced nucleus, which is supported by our experiments where we displaced the nucleus using optical tweezers
U2 - 10.1103/PhysRevLett.114.078103
DO - 10.1103/PhysRevLett.114.078103
M3 - Article
SN - 0031-9007
VL - 114
JO - Physical Review Letters
JF - Physical Review Letters
IS - 7
M1 - 078103
ER -