Isolation of differentially expressed genes from contaminant exposed European flounder by suppressive, subtractive hybridisation

Derek Sheader; Karl Gensberg; BP Lyons; James Chipman

doi:10.1016/j.marenvres.2004.03.044

Isolation of differentially expressed genes from contaminant exposed European flounder by suppressive, subtractive hybridisation

Derek Sheader, Karl Gensberg, BP Lyons, James Chipman

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Abstract

Commercially farmed European flounder (Platichthys flesus) were treated with benzo[a]pyrene or cadmium under controlled laboratory conditions. In addition, animals were collected from the polluted Tyne and relatively clean Alde estuaries in the UK. Potential differentially expressed genes were isolated from liver tissue by means of suppressive, subtractive hybridisation. A total of 284 different mRNAs were identified as having the potential for being differentially expressed, of which 84 could be tentatively identified by homology with database sequences. These clones will form part of a flounder cDNA array for validation and future applications, with full identification being pursued for those expressed sequence tags for which differential expression can be confirmed by array technology. (C) 2004 Published by Elsevier Ltd.

Original language	English
Pages (from-to)	553-557
Number of pages	5
Journal	Marine Environmental Research
Volume	58
DOIs	https://doi.org/10.1016/j.marenvres.2004.03.044
Publication status	Published - 1 Jan 2004

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title = "Isolation of differentially expressed genes from contaminant exposed European flounder by suppressive, subtractive hybridisation",

abstract = "Commercially farmed European flounder (Platichthys flesus) were treated with benzo[a]pyrene or cadmium under controlled laboratory conditions. In addition, animals were collected from the polluted Tyne and relatively clean Alde estuaries in the UK. Potential differentially expressed genes were isolated from liver tissue by means of suppressive, subtractive hybridisation. A total of 284 different mRNAs were identified as having the potential for being differentially expressed, of which 84 could be tentatively identified by homology with database sequences. These clones will form part of a flounder cDNA array for validation and future applications, with full identification being pursued for those expressed sequence tags for which differential expression can be confirmed by array technology. (C) 2004 Published by Elsevier Ltd.",

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doi = "10.1016/j.marenvres.2004.03.044",

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T1 - Isolation of differentially expressed genes from contaminant exposed European flounder by suppressive, subtractive hybridisation

AU - Sheader, Derek

AU - Gensberg, Karl

AU - Lyons, BP

AU - Chipman, James

PY - 2004/1/1

Y1 - 2004/1/1

N2 - Commercially farmed European flounder (Platichthys flesus) were treated with benzo[a]pyrene or cadmium under controlled laboratory conditions. In addition, animals were collected from the polluted Tyne and relatively clean Alde estuaries in the UK. Potential differentially expressed genes were isolated from liver tissue by means of suppressive, subtractive hybridisation. A total of 284 different mRNAs were identified as having the potential for being differentially expressed, of which 84 could be tentatively identified by homology with database sequences. These clones will form part of a flounder cDNA array for validation and future applications, with full identification being pursued for those expressed sequence tags for which differential expression can be confirmed by array technology. (C) 2004 Published by Elsevier Ltd.

AB - Commercially farmed European flounder (Platichthys flesus) were treated with benzo[a]pyrene or cadmium under controlled laboratory conditions. In addition, animals were collected from the polluted Tyne and relatively clean Alde estuaries in the UK. Potential differentially expressed genes were isolated from liver tissue by means of suppressive, subtractive hybridisation. A total of 284 different mRNAs were identified as having the potential for being differentially expressed, of which 84 could be tentatively identified by homology with database sequences. These clones will form part of a flounder cDNA array for validation and future applications, with full identification being pursued for those expressed sequence tags for which differential expression can be confirmed by array technology. (C) 2004 Published by Elsevier Ltd.

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DO - 10.1016/j.marenvres.2004.03.044

M3 - Article

VL - 58

SP - 553

EP - 557

JO - Marine Environmental Research

JF - Marine Environmental Research

ER -

Isolation of differentially expressed genes from contaminant exposed European flounder by suppressive, subtractive hybridisation

Abstract

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