Isolation and functional analysis of the histone H3 promoter from atlantic salmon (Salmo salar L.)

S Hanley, T J Smith, F Muller, N Maclean, S Uzbekova, P Prunet, B Breton

Research output: Contribution to journalArticlepeer-review

15 Citations (Scopus)

Abstract

The histone H3 (sH3) promoter of Atlantic salmon (Salmo salar) was cloned via polymerase chain reaction using primers designed from the rainbow trout (Oncorhynchus mykiss) promoter sequence. A comparison of the nucleotide sequence with the equivalent sequences from rainbow trout and sockeye salmon (Oncorhynchus nerka) revealed a high degree of conservation. In vivo expression analysis of the sH3 promoter was carried out in both rainbow trout and zebrafish (Danio rerio) embryos. A direct comparison of the sH3 promoter with the viral RSV promoter in rainbow trout resulted in stronger expression of the sH3 promoter. Furthermore, lacZ expression directed by the sH3 promoter was ubiquitous in several different cell types in developing zebrafish embryos. These results suggest that the sH3 promoter will be useful in transgenic studies in Atlantic salmon.

Original languageEnglish
Pages (from-to)165-72
Number of pages8
JournalMolecular marine biology and biotechnology
Volume7
Issue number3
Publication statusPublished - Sep 1998

Keywords

  • Animals
  • Base Sequence
  • Cloning, Molecular
  • DNA Primers
  • Embryo, Nonmammalian
  • Histones
  • Molecular Sequence Data
  • Oncorhynchus
  • Oncorhynchus mykiss
  • Polymerase Chain Reaction
  • Promoter Regions, Genetic
  • Recombinant Fusion Proteins
  • Salmon
  • Sequence Alignment
  • Sequence Homology, Nucleic Acid
  • Zebrafish
  • beta-Galactosidase

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