Isolation and characterization of the promoter and partial enhancer region of the porcine inter-alpha-trypsin inhibitor heavy chain 4 gene

Niamh Harraghy; Timothy J Mitchell

doi:10.1128/CDLI.12.11.1336-1339.2005

Isolation and characterization of the promoter and partial enhancer region of the porcine inter-alpha-trypsin inhibitor heavy chain 4 gene

Niamh Harraghy, Timothy J Mitchell

Microbiology and Infection

Research output: Contribution to journal › Article › peer-review

2 Citations (Scopus)

Abstract

A porcine genomic library was screened for clones containing the promoter of the major acute-phase protein in pigs, inter-alpha-trypsin heavy chain 4 (ITIH4). Following isolation of the promoter, a functional analysis was performed with Hep3B cells. The promoter was induced by interleukin-6 (IL-6) but not by IL-1beta. However, IL-1beta was shown to inhibit the IL-6-induced activation of the porcine ITIH4 promoter.

Original language	English
Pages (from-to)	1336-9
Number of pages	4
Journal	Clinical and Vaccine Immunology
Volume	12
Issue number	11
DOIs	https://doi.org/10.1128/CDLI.12.11.1336-1339.2005
Publication status	Published - Nov 2005

Keywords

Acute-Phase Proteins
Animals
Cell Line, Tumor
Enhancer Elements, Genetic
Gene Expression Regulation
Humans
Interleukin-1
Interleukin-6
Molecular Sequence Data
Promoter Regions, Genetic
Swine

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10.1128/CDLI.12.11.1336-1339.2005

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title = "Isolation and characterization of the promoter and partial enhancer region of the porcine inter-alpha-trypsin inhibitor heavy chain 4 gene",

abstract = "A porcine genomic library was screened for clones containing the promoter of the major acute-phase protein in pigs, inter-alpha-trypsin heavy chain 4 (ITIH4). Following isolation of the promoter, a functional analysis was performed with Hep3B cells. The promoter was induced by interleukin-6 (IL-6) but not by IL-1beta. However, IL-1beta was shown to inhibit the IL-6-induced activation of the porcine ITIH4 promoter.",

keywords = "Acute-Phase Proteins, Animals, Cell Line, Tumor, Enhancer Elements, Genetic, Gene Expression Regulation, Humans, Interleukin-1, Interleukin-6, Molecular Sequence Data, Promoter Regions, Genetic, Swine",

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year = "2005",

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doi = "10.1128/CDLI.12.11.1336-1339.2005",

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T1 - Isolation and characterization of the promoter and partial enhancer region of the porcine inter-alpha-trypsin inhibitor heavy chain 4 gene

AU - Harraghy, Niamh

AU - Mitchell, Timothy J

PY - 2005/11

Y1 - 2005/11

N2 - A porcine genomic library was screened for clones containing the promoter of the major acute-phase protein in pigs, inter-alpha-trypsin heavy chain 4 (ITIH4). Following isolation of the promoter, a functional analysis was performed with Hep3B cells. The promoter was induced by interleukin-6 (IL-6) but not by IL-1beta. However, IL-1beta was shown to inhibit the IL-6-induced activation of the porcine ITIH4 promoter.

AB - A porcine genomic library was screened for clones containing the promoter of the major acute-phase protein in pigs, inter-alpha-trypsin heavy chain 4 (ITIH4). Following isolation of the promoter, a functional analysis was performed with Hep3B cells. The promoter was induced by interleukin-6 (IL-6) but not by IL-1beta. However, IL-1beta was shown to inhibit the IL-6-induced activation of the porcine ITIH4 promoter.

KW - Acute-Phase Proteins

KW - Animals

KW - Cell Line, Tumor

KW - Enhancer Elements, Genetic

KW - Gene Expression Regulation

KW - Humans

KW - Interleukin-1

KW - Interleukin-6

KW - Molecular Sequence Data

KW - Promoter Regions, Genetic

KW - Swine

U2 - 10.1128/CDLI.12.11.1336-1339.2005

DO - 10.1128/CDLI.12.11.1336-1339.2005

M3 - Article

C2 - 16275952

SN - 1556-6811

VL - 12

SP - 1336

EP - 1339

JO - Clinical and Vaccine Immunology

JF - Clinical and Vaccine Immunology

IS - 11

ER -

Isolation and characterization of the promoter and partial enhancer region of the porcine inter-alpha-trypsin inhibitor heavy chain 4 gene

Abstract

Keywords

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