Investigation of the Pseudomonas aeruginosa ampR gene and its role at the chromosomal ampC beta-lactamase promoter

J Lodge, S Busby, L Piddock

Research output: Contribution to journalArticlepeer-review

38 Citations (Scopus)

Abstract

The complete sequence of the Pseudomonas aeruginosa beta-lactamase regulator gene ampR has been determined. The amino acid sequence encoded by this gene is compared with other members of the LysR family of transcription factors. The P. aeruginosa ampC promoter was subcloned and gel mobility shift assays were used to demonstrate specific binding of AmpR to the ampC promoter region.
Original languageEnglish
Pages (from-to)315-20
Number of pages6
JournalFEMS Microbiology Letters
Volume111
Issue number2-3
Publication statusPublished - 1 Aug 1993

Keywords

  • Amino Acid Sequence
  • Bacterial Proteins
  • Base Sequence
  • Chromosomes, Bacterial
  • Consensus Sequence
  • DNA, Bacterial
  • Genes, Bacterial
  • Genes, Regulator
  • Molecular Sequence Data
  • Promoter Regions, Genetic
  • Pseudomonas aeruginosa
  • Restriction Mapping
  • Sequence Homology, Amino Acid
  • Transcription Factors
  • beta-Lactamases

Fingerprint

Dive into the research topics of 'Investigation of the Pseudomonas aeruginosa ampR gene and its role at the chromosomal ampC beta-lactamase promoter'. Together they form a unique fingerprint.

Cite this