Investigating cell membrane structure and dynamics with TCSPC-FLIM

Alix Le Marois; Dylan M. Owen; Klaus Suhling

doi:10.1117/12.2178563

Investigating cell membrane structure and dynamics with TCSPC-FLIM

Alix Le Marois, Dylan M. Owen, Klaus Suhling

Immunology and Immunotherapy

Research output: Chapter in Book/Report/Conference proceeding › Chapter

1 Citation (Scopus)

Abstract

We report the use of Time-Correlated Single Photon Counting (TCSPC) in a polarization-resolved Fluorescence Lifetime Imaging (FLIM) setup for the investigation of cell membrane structural and dynamic properties. This technique allows us to study the orientation and mobility of fluorescent membrane dyes, namely di-4-ANEPPDHQ and DiO, in model bilayers of different lipid compositions. Dipole alignment and extent of rotational motion can be linked to membrane order and fluidity. Comparison of the time-resolved anisotropy decays of the two fluorescent dyes suggests that rotational motion of membrane constituents is restricted in liquid-ordered phases, and appears to be limited to the region of aliphatic tails in liquid-disordered phases. In living cells, understanding the membrane structure provides crucial information on its functional properties, such as exo- and endocytosis, cell mobility and signal transduction.

Original language	English
Title of host publication	Progress in Biomedical Optics and Imaging - Proceedings of SPIE
Publisher	Society of Photo-Optical Instrumentation Engineers
Volume	9329
ISBN (Print)	9781628414196
DOIs	https://doi.org/10.1117/12.2178563
Publication status	Published - 5 Mar 2015

Keywords

liquid-ordered and disordered phases
membrane order
TCSPC
Time-resolved Fluorescence Anisotropy Imaging

Access to Document

10.1117/12.2178563

Cite this

@inbook{d7416c5e439944d6b22c8e1d1059b048,

title = "Investigating cell membrane structure and dynamics with TCSPC-FLIM",

abstract = "We report the use of Time-Correlated Single Photon Counting (TCSPC) in a polarization-resolved Fluorescence Lifetime Imaging (FLIM) setup for the investigation of cell membrane structural and dynamic properties. This technique allows us to study the orientation and mobility of fluorescent membrane dyes, namely di-4-ANEPPDHQ and DiO, in model bilayers of different lipid compositions. Dipole alignment and extent of rotational motion can be linked to membrane order and fluidity. Comparison of the time-resolved anisotropy decays of the two fluorescent dyes suggests that rotational motion of membrane constituents is restricted in liquid-ordered phases, and appears to be limited to the region of aliphatic tails in liquid-disordered phases. In living cells, understanding the membrane structure provides crucial information on its functional properties, such as exo- and endocytosis, cell mobility and signal transduction.",

keywords = "liquid-ordered and disordered phases, membrane order, TCSPC, Time-resolved Fluorescence Anisotropy Imaging",

author = "{Le Marois}, Alix and Owen, {Dylan M.} and Klaus Suhling",

year = "2015",

month = mar,

day = "5",

doi = "10.1117/12.2178563",

language = "English",

isbn = "9781628414196",

volume = "9329",

booktitle = "Progress in Biomedical Optics and Imaging - Proceedings of SPIE",

publisher = "Society of Photo-Optical Instrumentation Engineers",

address = "United States",

}

TY - CHAP

T1 - Investigating cell membrane structure and dynamics with TCSPC-FLIM

AU - Le Marois, Alix

AU - Owen, Dylan M.

AU - Suhling, Klaus

PY - 2015/3/5

Y1 - 2015/3/5

N2 - We report the use of Time-Correlated Single Photon Counting (TCSPC) in a polarization-resolved Fluorescence Lifetime Imaging (FLIM) setup for the investigation of cell membrane structural and dynamic properties. This technique allows us to study the orientation and mobility of fluorescent membrane dyes, namely di-4-ANEPPDHQ and DiO, in model bilayers of different lipid compositions. Dipole alignment and extent of rotational motion can be linked to membrane order and fluidity. Comparison of the time-resolved anisotropy decays of the two fluorescent dyes suggests that rotational motion of membrane constituents is restricted in liquid-ordered phases, and appears to be limited to the region of aliphatic tails in liquid-disordered phases. In living cells, understanding the membrane structure provides crucial information on its functional properties, such as exo- and endocytosis, cell mobility and signal transduction.

AB - We report the use of Time-Correlated Single Photon Counting (TCSPC) in a polarization-resolved Fluorescence Lifetime Imaging (FLIM) setup for the investigation of cell membrane structural and dynamic properties. This technique allows us to study the orientation and mobility of fluorescent membrane dyes, namely di-4-ANEPPDHQ and DiO, in model bilayers of different lipid compositions. Dipole alignment and extent of rotational motion can be linked to membrane order and fluidity. Comparison of the time-resolved anisotropy decays of the two fluorescent dyes suggests that rotational motion of membrane constituents is restricted in liquid-ordered phases, and appears to be limited to the region of aliphatic tails in liquid-disordered phases. In living cells, understanding the membrane structure provides crucial information on its functional properties, such as exo- and endocytosis, cell mobility and signal transduction.

KW - liquid-ordered and disordered phases

KW - membrane order

KW - TCSPC

KW - Time-resolved Fluorescence Anisotropy Imaging

U2 - 10.1117/12.2178563

DO - 10.1117/12.2178563

M3 - Chapter

SN - 9781628414196

VL - 9329

BT - Progress in Biomedical Optics and Imaging - Proceedings of SPIE

PB - Society of Photo-Optical Instrumentation Engineers

ER -

Investigating cell membrane structure and dynamics with TCSPC-FLIM

Abstract

Keywords

Access to Document

Fingerprint

Cite this