Intrathymic administration of B cells induces prolonged survival of fully allogeneic cardiac grafts without prolonged deletion of donor-specific thymocytes

M Niimi, N D Jones, O B Pajaro, P J Morris, K J Wood

Research output: Contribution to journalArticlepeer-review

13 Citations (Scopus)

Abstract

Intrathymic (IT) injection of alloantigen has been shown to induce unresponsiveness to allografts although the exact mechanisms of tolerance induction remains unclear. C57BL/10 (H2b) cardiac allografts were accepted in C3H/He (H2k) mice pretreated with IT inoculation of donor splenocytes (1 x 10(6)) in combination with a depleting anti-CD4 monoclonal antibody 27 days before cardiac transplantation. To investigate which cell types were responsible for tolerance induction by IT injection of alloantigen, resting B (rB) cells or dendritic cells were used as the thymic inoculum instead of whole splenocytes. IT injection of rB cells induced indefinite graft prolongation in all recipients while only 20% of mice that had received IT injection of dendritic cells accepted grafts for over 100 days. In contrast, IT injection of dendritic cells resulted in significant deletion of donor-specific thymocytes whereas rB cells were relatively ineffective. IT deletion is not essential for the induction of tolerance by IT injection of rB cells; nondeletional mechanisms can be involved.
Original languageEnglish
Pages (from-to)177-81
Number of pages5
JournalTransplant Immunology
Volume6
Issue number3
Publication statusPublished - Sep 1998

Keywords

  • Animals
  • Heart Transplantation
  • Thymus Gland
  • Graft Survival
  • Mice, Inbred C57BL
  • Mice, Inbred C3H
  • Mice
  • Transplantation, Homologous
  • Mice, Inbred BALB C
  • B-Lymphocytes
  • Time Factors
  • Tissue Donors

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