Intracellular processing of transferrin and iron by isolated rat hepatocytes

S P Young, S Roberts, A Bomford

Research output: Contribution to journalArticlepeer-review

31 Citations (Scopus)


Transferrin bound by isolated rat hepatocytes is rapidly endocytosed and enters a compartment of low density. Little was found associated with the lysosomes, even though the protein was subsequently lost from the cells. Iron entering the cells on transferrin was subsequently found in a number of intracellular components: transferrin, haem, ferritin and a residual fraction. After 2 h incubation with 59Fe-transferrin almost 70% of the iron was in ferritin, and this proportion increased to 80% during a 'chase' experiment. Residual iron, because of its rapid increase at the start of the incubation and its decline during the 'chase', probably represents an intracellular transit pool, which at steady state was present at 23 pg/10(6) cells.
Original languageEnglish
Pages (from-to)819-23
Number of pages5
JournalBiochemical Journal
Issue number3
Publication statusPublished - 15 Dec 1985


  • Asialoglycoproteins
  • Centrifugation, Density Gradient
  • Animals
  • Cell Fractionation
  • Rats
  • Rats, Inbred Strains
  • Endocytosis
  • Transferrin
  • Intracellular Fluid
  • Liver
  • Orosomucoid
  • Iron
  • Male


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