Interacting effects of Ca2+ and hypoxia in the induction of sarcolemmal damage in mouse diaphragm in vitro

N J Bannister, S J Publicover

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4 Citations (Scopus)


Experiments have been undertaken to investigate the basis for the selective damage of centrally placed fibres in mouse diaphragms exposed to Ca2+ loading in vitro. Incubation under hypoxic conditions (non-aerated saline) for 30 min had no discernible effect on the muscle. Incubation for 120 min led to permeabilisation of the sarcolemma (assayed by penetration of Procion Yellow) in 54% of cells. Sarcolemmal permeabilisation was almost completely restricted to centrally placed cells, as has previously been described for the effects of the Ca2+ channel agonist Bay K 8644. Ultrastructural damage to the myofibrils and mitochondrial swelling were also widespread amongst centrally placed cells. Permeabilisation was inhibited when hypoxic incubations were carried out in Ca(2+)-free saline. Incubation in hypoxic Ca(2+)-containing saline for 30 min followed by further incubation in Ca(2+)-free, hypoxic saline, up to a total of 120 min, resulted in permeabilisation similar to that seen in muscles incubated for 120 min in Ca(2+)-containing, hypoxic saline. It is suggested that selective damage to central fibres induced by Ca2+ loading resulting from treatment with Bay K 8644 is related to increased oxygen demand.
Original languageEnglish
Pages (from-to)411-4
Number of pages4
JournalActa Neuropathologica
Issue number4
Publication statusPublished - 1995


  • Animals
  • Calcium
  • Diaphragm
  • Calcium Channel Agonists
  • Sarcolemma
  • 3-Pyridinecarboxylic acid, 1,4-dihydro-2,6-dimethyl-5-nitro-4-(2-(trifluoromethyl)phenyl)-, Methyl ester
  • Mice
  • Mice, Inbred BALB C
  • Male
  • Female
  • Anoxia


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