TY - JOUR
T1 - Insights into the ancestry evolution of the Mycobacterium tuberculosis complex from analysis of Mycobacterium riyadhense
AU - Guan, Qingtian
AU - Garbati, Musa
AU - Mfarrej, Sara
AU - AlMutairi, Talal
AU - Laval, Thomas
AU - Singh, Albel
AU - Fagbo, Shamsudeen
AU - Smyth, Alicia
AU - Browne, John A
AU - urRahman, Muhammad Amin
AU - Alruwaili, Alya
AU - Hoosen, Anwar
AU - Meehan, Conor J
AU - Nakajima, Chie
AU - Suzuki, Yasuhiko
AU - Demangel, Caroline
AU - Bhatt, Apoorva
AU - Gordon, Stephen V
AU - AlAsmari, Faisal
AU - Pain, Arnab
N1 - © The Author(s) 2021. Published by Oxford University Press on behalf of NAR Genomics and Bioinformatics.
PY - 2021/9
Y1 - 2021/9
N2 - Current evolutionary scenarios posit the emergence of Mycobacterium tuberculosis from an environmental saprophyte through a cumulative process of genome adaptation. Mycobacterium riyadhense, a related bacillus, is being increasingly isolated from human clinical cases with tuberculosis-like symptoms in various parts of the world. To elucidate the evolutionary relationship between M. riyadhense and other mycobacterial species, including members of the M. tuberculosis complex (MTBC), eight clinical isolates of M. riyadhense were sequenced and analyzed. We show, among other features, that M. riyadhense shares a large number of conserved orthologs with M. tuberculosis and shows the expansion of toxin/antitoxin pairs, PE/PPE family proteins compared with other non-tuberculous mycobacteria. We observed M. riyadhense lacks wecE gene which may result in the absence of lipooligosaccharides (LOS) IV. Comparative transcriptomic analysis of infected macrophages reveals genes encoding inducers of Type I IFN responses, such as cytosolic DNA sensors, were relatively less expressed by macrophages infected with M. riyadhense or M. kansasii, compared to BCG or M. tuberculosis. Overall, our work sheds new light on the evolution of M. riyadhense, its relationship to the MTBC, and its potential as a system for the study of mycobacterial virulence and pathogenesis.
AB - Current evolutionary scenarios posit the emergence of Mycobacterium tuberculosis from an environmental saprophyte through a cumulative process of genome adaptation. Mycobacterium riyadhense, a related bacillus, is being increasingly isolated from human clinical cases with tuberculosis-like symptoms in various parts of the world. To elucidate the evolutionary relationship between M. riyadhense and other mycobacterial species, including members of the M. tuberculosis complex (MTBC), eight clinical isolates of M. riyadhense were sequenced and analyzed. We show, among other features, that M. riyadhense shares a large number of conserved orthologs with M. tuberculosis and shows the expansion of toxin/antitoxin pairs, PE/PPE family proteins compared with other non-tuberculous mycobacteria. We observed M. riyadhense lacks wecE gene which may result in the absence of lipooligosaccharides (LOS) IV. Comparative transcriptomic analysis of infected macrophages reveals genes encoding inducers of Type I IFN responses, such as cytosolic DNA sensors, were relatively less expressed by macrophages infected with M. riyadhense or M. kansasii, compared to BCG or M. tuberculosis. Overall, our work sheds new light on the evolution of M. riyadhense, its relationship to the MTBC, and its potential as a system for the study of mycobacterial virulence and pathogenesis.
U2 - 10.1093/nargab/lqab070
DO - 10.1093/nargab/lqab070
M3 - Article
C2 - 34396095
SN - 2631-9268
VL - 3
SP - lqab070
JO - NAR genomics and bioinformatics
JF - NAR genomics and bioinformatics
IS - 3
ER -