In vitro modelling of disease-induced changes in the diabetic wound fibroblast

Parviz Sorooshian, Anthony D Metcalfe, Ferdinand V Lali

Research output: Contribution to journalArticlepeer-review

Abstract

OBJECTIVE: Fibroblasts have been shown to play an increasingly important role within diabetic wounds. While several in vitro models of diabetic wound fibroblasts have been reported, none replicate the natural progression of the disease over time, recapitulating the acquisition of the diseased phenotype. Therefore, this study aimed to establish an in vitro model of the diabetic wound fibroblast through sustained exposure of healthy dermal fibroblasts to hyperglycaemia.

METHOD: Primary human fibroblasts were isolated from discarded healthy skin tissue and were either exposed to normoglycaemic (control 5.5mM glucose) media or hyperglycaemic (25mM glucose) media for four weeks. Quantitative polymerase chain reaction was performed to measure the expression of inflammatory cytokines and chemokines.

RESULTS: In the hyperglycaemia model, stromal cell-derived factor (SDF)-1 expression remained consistently downregulated across all four weeks (p<0.01), while monocyte chemoattractant protein (MCP)-1 (p<0.001), interleukin (IL)-8 (p=0.847) and chemokine (C-X-C motif) ligand 1 (CXCL1) (p=0.872) were initially downregulated at one week followed by subsequent upregulation between 2-4 weeks.

CONCLUSION: This hyperglycaemia model may serve as a useful tool to characterise pathological changes in the diabetic wound fibroblast and help identify candidate therapeutic targets, such as SDF-1, that may reverse the pathology.

Original languageEnglish
Pages (from-to)300-303
Number of pages4
JournalJournal of Wound Care
Volume30
Issue number4
DOIs
Publication statusPublished - 2 Apr 2021

Keywords

  • Chemokines
  • Cytokines
  • Diabetes Complications
  • Diabetes Mellitus
  • Diabetic Foot/therapy
  • Fibroblasts
  • Humans
  • Skin/pathology
  • Wound Healing/physiology

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