In vitro degradation and in vivo resorption of dicalcium phosphate cement based grafts

Zeeshan Sheikh, Yu Ling Zhang, Liam Grover, Géraldine E. Merle, Faleh Tamimi, Jake Barralet

Research output: Contribution to journalArticlepeer-review

47 Citations (Scopus)
228 Downloads (Pure)

Abstract

There are two types of DCP: dihydrated (brushite) and anhydrous (monetite). After implantation, brushite converts to hydroxyapatite (HA) which resorbs very slowly. This conversion is not observed after implantation of monetite cements and result in a greater of resorption. The precise mechanisms of resorption and degradation however of these ceramics remain uncertain. This study was designed to investigate the effect of: porosity, surface area and hydration on in vitro degradation and in vivo resorption of DCP. Brushite and two types of monetite cement based grafts (produced by wet and dry thermal conversion) were aged in phosphate buffered saline (PBS) and bovine serum solutions in vitro and were implanted subcutaneously in rats. Here we show that for high relative porosity grafts (50-65%), solubility and surface area does not play a significant role towards in vitro mass loss with disintegration and fragmentation being the main factors dictating mass loss. For grafts having lower relative porosity (35-45%), solubility plays a more crucial role in mass loss during in vitro ageing and in vivo resorption. Also, serum inhibited dissolution and the formation of HA in brushite cements. However, when aged in PBS, brushite undergoes phase conversion to a mixture of octacalcium phosphate (OCP) and HA. This phase conversion was not observed for monetite upon ageing (in both serum and PBS) or in subcutaneous implantation. This study provides greater understanding of the degradation and resorption process of DCP based grafts, allowing us to prepare bone replacement materials with more predictable resorption profiles.

Original languageEnglish
Pages (from-to)338-346
Number of pages9
JournalActa Biomaterialia
Volume26
Early online date20 Aug 2015
DOIs
Publication statusPublished - 15 Oct 2015

Keywords

  • Brushite
  • Cements
  • Dicalcium phosphate
  • In vitro degradation
  • In vivo resorption
  • Monetite

ASJC Scopus subject areas

  • Biomaterials
  • Biomedical Engineering
  • Biotechnology
  • Biochemistry
  • Molecular Biology

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