TY - JOUR
T1 - In situ SERS probing of nano-silver coated individual yeast cells
AU - Chrimes, A.F.
AU - Khoshmanesh, K.
AU - Tang, S.-Y.
AU - Wood, B.R.
AU - Stoddart, P.R.
AU - Collins, S.S.E.
AU - Mitchell, A.
AU - Kalantar-zadeh, K.
PY - 2013/11/15
Y1 - 2013/11/15
N2 - For understanding cells functionalities and their communications, there is a need for highly sensitive cell analysis platforms capable of assessing non-specific chemicals on the surface and in the vicinity of cells. We report a microfluidic system integrating dielectrophoresis and surface enhanced Raman scattering (SERS) for the trapping and real time monitoring of cell functions in isolated and grouped cell clusters. Yeast cells are coated with silver nanoparticles to enable highly sensitive SERS analysis. The SERS responses of cells are examined under various conditions: live vs. dead and isolated vs. grouped. This work illustrates the feasibility of the system for in situ cell monitoring and analysis of secreted chemicals during their growth, metabolism, proliferation and apoptosis.
AB - For understanding cells functionalities and their communications, there is a need for highly sensitive cell analysis platforms capable of assessing non-specific chemicals on the surface and in the vicinity of cells. We report a microfluidic system integrating dielectrophoresis and surface enhanced Raman scattering (SERS) for the trapping and real time monitoring of cell functions in isolated and grouped cell clusters. Yeast cells are coated with silver nanoparticles to enable highly sensitive SERS analysis. The SERS responses of cells are examined under various conditions: live vs. dead and isolated vs. grouped. This work illustrates the feasibility of the system for in situ cell monitoring and analysis of secreted chemicals during their growth, metabolism, proliferation and apoptosis.
UR - http://www.scopus.com/inward/record.url?eid=2-s2.0-84880148916&partnerID=MN8TOARS
U2 - 10.1016/j.bios.2013.05.053
DO - 10.1016/j.bios.2013.05.053
M3 - Article
SN - 0956-5663
VL - 49
SP - 536
EP - 541
JO - Biosensors and Bioelectronics
JF - Biosensors and Bioelectronics
M1 - 536-541
ER -