Importance of Toxin A, Toxin B, and CDT in virulence of an epidemic Clostridium difficile strain

Sarah Kuehne; Mark M Collery; Michelle L Kelly; Stephen T Cartman; Alan Cockayne; Nigel P Minton

doi:10.1093/infdis/jit426

Importance of Toxin A, Toxin B, and CDT in virulence of an epidemic Clostridium difficile strain

Sarah Kuehne, Mark M Collery, Michelle L Kelly, Stephen T Cartman, Alan Cockayne, Nigel P Minton

Dentistry

Research output: Contribution to journal › Article › peer-review

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Abstract

Clostridium difficile infection is the main cause of healthcare-acquired diarrhea in the developed world. In addition to the main virulence factors toxin A and B, epidemic, PCR Ribotype 027 strains, such as R20291, produce a third toxin, CDT. To develop effective medical countermeasures, it is important to understand the importance of each toxin. Accordingly, we created all possible combinations of isogenic toxin mutants of R20291 and assessed their virulence. We demonstrated that either toxin A or toxin B alone can cause fulminant disease in the hamster infection model and present tantalizing data that C. difficile toxin may also contribute to virulence.

Original language	English
Pages (from-to)	83-86
Number of pages	4
Journal	The Journal of Infectious Diseases
Volume	209
Issue number	1
Early online date	8 Aug 2013
DOIs	https://doi.org/10.1093/infdis/jit426
Publication status	Published - 1 Jan 2014

Keywords

ADP Ribose Transferases
Animals
Bacterial Proteins
Bacterial Toxins
Cell Death
Clostridium Infections
Clostridium difficile
Cricetinae
Enterotoxins
Female
HT29 Cells
Humans
Mesocricetus
Virulence
Journal Article
Research Support, Non-U.S. Gov't
Clostridium difficile infection
TcdA
TcdB
pathogenesis
CDT

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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KuehneS2013ImportanceFinal published version, 194 KBLicence: Creative Commons: Attribution (CC BY)

https://doi.org/10.1093/infdis/jit426Licence: Creative Commons: Attribution (CC BY)

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title = "Importance of Toxin A, Toxin B, and CDT in virulence of an epidemic Clostridium difficile strain",

abstract = "Clostridium difficile infection is the main cause of healthcare-acquired diarrhea in the developed world. In addition to the main virulence factors toxin A and B, epidemic, PCR Ribotype 027 strains, such as R20291, produce a third toxin, CDT. To develop effective medical countermeasures, it is important to understand the importance of each toxin. Accordingly, we created all possible combinations of isogenic toxin mutants of R20291 and assessed their virulence. We demonstrated that either toxin A or toxin B alone can cause fulminant disease in the hamster infection model and present tantalizing data that C. difficile toxin may also contribute to virulence.",

keywords = "ADP Ribose Transferases, Animals, Bacterial Proteins, Bacterial Toxins, Cell Death, Clostridium Infections, Clostridium difficile, Cricetinae, Enterotoxins, Female, HT29 Cells, Humans, Mesocricetus, Virulence, Journal Article, Research Support, Non-U.S. Gov't, Clostridium difficile infection, TcdA, TcdB, pathogenesis, CDT",

author = "Sarah Kuehne and Collery, {Mark M} and Kelly, {Michelle L} and Cartman, {Stephen T} and Alan Cockayne and Minton, {Nigel P}",

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doi = "10.1093/infdis/jit426",

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journal = "The Journal of Infectious Diseases",

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T1 - Importance of Toxin A, Toxin B, and CDT in virulence of an epidemic Clostridium difficile strain

AU - Kuehne, Sarah

AU - Collery, Mark M

AU - Kelly, Michelle L

AU - Cartman, Stephen T

AU - Cockayne, Alan

AU - Minton, Nigel P

PY - 2014/1/1

Y1 - 2014/1/1

N2 - Clostridium difficile infection is the main cause of healthcare-acquired diarrhea in the developed world. In addition to the main virulence factors toxin A and B, epidemic, PCR Ribotype 027 strains, such as R20291, produce a third toxin, CDT. To develop effective medical countermeasures, it is important to understand the importance of each toxin. Accordingly, we created all possible combinations of isogenic toxin mutants of R20291 and assessed their virulence. We demonstrated that either toxin A or toxin B alone can cause fulminant disease in the hamster infection model and present tantalizing data that C. difficile toxin may also contribute to virulence.

AB - Clostridium difficile infection is the main cause of healthcare-acquired diarrhea in the developed world. In addition to the main virulence factors toxin A and B, epidemic, PCR Ribotype 027 strains, such as R20291, produce a third toxin, CDT. To develop effective medical countermeasures, it is important to understand the importance of each toxin. Accordingly, we created all possible combinations of isogenic toxin mutants of R20291 and assessed their virulence. We demonstrated that either toxin A or toxin B alone can cause fulminant disease in the hamster infection model and present tantalizing data that C. difficile toxin may also contribute to virulence.

KW - ADP Ribose Transferases

KW - Animals

KW - Bacterial Proteins

KW - Bacterial Toxins

KW - Cell Death

KW - Clostridium Infections

KW - Clostridium difficile

KW - Cricetinae

KW - Enterotoxins

KW - Female

KW - HT29 Cells

KW - Humans

KW - Mesocricetus

KW - Virulence

KW - Journal Article

KW - Research Support, Non-U.S. Gov't

KW - Clostridium difficile infection

KW - TcdA

KW - TcdB

KW - pathogenesis

KW - CDT

U2 - 10.1093/infdis/jit426

DO - 10.1093/infdis/jit426

M3 - Article

C2 - 23935202

SN - 0022-1899

VL - 209

SP - 83

EP - 86

JO - The Journal of Infectious Diseases

JF - The Journal of Infectious Diseases

IS - 1

ER -

Importance of Toxin A, Toxin B, and CDT in virulence of an epidemic Clostridium difficile strain

Abstract

Keywords

UN SDGs

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