Importance of Micromonospora spp. as colonizers of cellulose in freshwater lakes as demonstrated by quantitative reverse transcriptase PCR of 16S rRNA

Alexandre B. de Menezes, James E McDonald, Heather E. Allison, Alan J. McCarthy

Research output: Contribution to journalArticlepeer-review

Abstract

The relative abundance of micromonosporas in the bacterial communities inhabiting cellulose baits, water columns, and sediments of two freshwater lakes was determined by quantitative PCR (qPCR) of reverse-transcribed 16S rRNA. Micromonospora spp. were shown to be significant members of the active bacterial population colonizing cellulosic substrates in the lake sediment, and their increased prevalence with greater depth was confirmed by enumeration of CFU.
Original languageEnglish
Pages (from-to)3495-3499
Number of pages5
JournalApplied and Environmental Microbiology
Volume78
Issue number9
DOIs
Publication statusPublished - May 2012

Keywords

  • Bacterial Load
  • Cellulose
  • DNA, Bacterial
  • DNA, Ribosomal
  • Fresh Water
  • Geologic Sediments
  • Micromonospora
  • Molecular Sequence Data
  • RNA, Ribosomal, 16S
  • Reverse Transcriptase Polymerase Chain Reaction
  • Sequence Analysis, DNA
  • Journal Article

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