Impaired sphingosine-1-phosphate synthesis induces preeclampsia by deactivating trophoblastic YAP (yes-associated protein) through S1PR2 (sphingosine-1-phosphate receptor-2)-induced actin polymerizations

Jiujiang Liao, Yangxi Zheng, Mingyu Hu, Ping Xu, Li Lin, Xiyao Liu, Yue Wu, Biao Huang, Xuan Ye, Sisi Li, Ran Duan, Huijia Fu, Jiayu Huang, Li Wen, Yong Fu, Mark Kilby, Louise C Kenny, Philip N Baker, Hongbo Qi, Chao Tong

Research output: Contribution to journalArticlepeer-review

99 Downloads (Pure)

Abstract

Incomplete spiral artery remodeling, caused by impaired extravillous trophoblast invasion, is a fundamental pathogenic process associated with malplacentation and the development of preeclampsia. Nevertheless, the mechanisms controlling this regulation of trophoblast invasion are largely unknown. We report that sphingosine-1-phosphate synthesis and expression is abundant in healthy trophoblast, whereas in pregnancies complicated by preeclampsia the placentae are associated with reduced sphingosine-1-phosphate and lower SPHK1 (sphingosine kinase 1) expression and activity. In vivo inhibition of sphingosine kinase 1 activity during placentation in pregnant mice led to decreased placental sphingosine-1-phosphate production and defective placentation, resulting in a preeclampsia phenotype. Moreover, sphingosine-1-phosphate increased HTR8/SVneo (immortalized human trophoblst cells) cell invasion in a Hippo-signaling–dependent transcriptional coactivator YAP (Yes-associated protein) dependent manner, which is activated by S1PR2 (sphingosine-1-phosphate receptor-2) and downstream RhoA (Ras homolog gene family, member A)/ROCK (Rho-associated protein kinase) induced actin polymerization. Mutation-based YAP-5SA (S61A, S109A, S127A, S164A, S381A) demonstrated that sphingosine-1-phosphate activation of YAP could be either dependent or independent of Hippo signaling. Together, these findings suggest a novel pathogenic pathway of preeclampsia via disrupted sphingosine-1-phosphate metabolism and signaling-induced, interrupted actin dynamics and YAP deactivation; this may lead to potential novel intervention targets for the prevention and management of preeclampsia.

Original languageEnglish
Pages (from-to)399–412
Number of pages14
JournalHypertension
Volume79
Issue number2
Early online date6 Dec 2021
DOIs
Publication statusPublished - Feb 2022

Bibliographical note

Funding Information:
This study was funded by National Key R&D Program of China (2018YFC1004103), National Natural Science Foundation of China (81671488, 81771613, 81871189, and 82071675).

Publisher Copyright:
© 2021 American Heart Association, Inc.

Keywords

  • Cytoskeleton
  • Preeclampsia
  • Pregnancy
  • Sphingosine
  • Trophoblasts

ASJC Scopus subject areas

  • Internal Medicine

Fingerprint

Dive into the research topics of 'Impaired sphingosine-1-phosphate synthesis induces preeclampsia by deactivating trophoblastic YAP (yes-associated protein) through S1PR2 (sphingosine-1-phosphate receptor-2)-induced actin polymerizations'. Together they form a unique fingerprint.

Cite this