Projects per year
Abstract
Although protein hydroxylation is a relatively poorly characterized posttranslational modification, it has received significant recent attention following seminal work uncovering its role in oxygen sensing and hypoxia biology. Although the fundamental importance of protein hydroxylases in biology is becoming clear, the biochemical targets and cellular functions often remain enigmatic. JMJD5 is a "JmjC-only" protein hydroxylase that is essential for murine embryonic development and viability. However, no germline variants in JmjC-only hydroxylases, including JMJD5, have yet been described that are associated with any human pathology. Here we demonstrate that biallelic germline JMJD5 pathogenic variants are deleterious to JMJD5 mRNA splicing, protein stability, and hydroxylase activity, resulting in a human developmental disorder characterized by severe failure to thrive, intellectual disability, and facial dysmorphism. We show that the underlying cellular phenotype is associated with increased DNA replication stress and that this is critically dependent on the protein hydroxylase activity of JMJD5. This work contributes to our growing understanding of the role and importance of protein hydroxylases in human development and disease.
Original language | English |
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Article number | e152784 |
Journal | Journal of Clinical Investigation |
Volume | 133 |
Issue number | 7 |
Early online date | 16 Feb 2023 |
DOIs | |
Publication status | Published - 3 Apr 2023 |
Keywords
- Humans
- Animals
- Mice
- Histone Demethylases/genetics
- Mixed Function Oxygenases/genetics
- Protein Processing, Post-Translational
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Dive into the research topics of 'Impaired protein hydroxylase activity causes replication stress and developmental abnormalities in humans'. Together they form a unique fingerprint.Projects
- 2 Active
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Investigating the Role of Protein hydroxylation in Cancer (studentship)
Saponaro, M. (Co-Investigator), Higgs, M. (Co-Investigator), Coleman, M. (Principal Investigator), Stewart, G. (Co-Investigator) & Beggs, A. (Co-Investigator)
1/10/18 → 31/12/24
Project: Research
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Characterising novel regulators of the PI-3-kinase-like kinase-dependent DNA damage response and their role in preventing human disease and cancer
Stewart, G. (Principal Investigator)
1/09/17 → 31/05/25
Project: Research