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Abstract
Aim: To investigate the impact of weight loss following gastric band surgery on multiple measures of peripheral blood neutrophil (PBN) function.
Material & Methods: Twenty-three obese patients undergoing gastric band surgery were recruited to a longitudinal intervention study, alongside non-obese, healthy gender and age-matched controls. Eighteen pairs of patients and controls completed all stages of the study. Peripheral blood neutrophils (PBN) were isolated by density centrifugation and a comprehensive analysis of PBN function undertaken at various stages of the patients’ bariatric surgical care pathway.
Results: Obese patients exhibited exaggerated PBN activity in response to various stimuli, characterised by higher reactive oxygen species (ROS) generation (n=18, p<0.001) and release of pro-inflammatory cytokines (n=10, p<0.05), and lower PBN extracellular trap (NET) formation (n=18, p<0.01). PBN chemotactic accuracy was also impaired prior to surgery (n=18, p<0.01). Weight loss was associated with normalised NET production and lower ROS production and cytokine release relative to healthy controls. However, chemotactic accuracy remained impaired in patients.
Conclusions: Weight loss following gastric band surgery was associated with a decrease in the pro-inflammatory activities of peripheral blood neutrophils (PBNs). A hyper-inflammatory PBN phenotype, involving excess ROS and cytokine release, reduced NET formation and chemotaxis, may lead to a reduced ability to eliminate infection, along-side inflammation-mediated tissue damage in obese individuals.
Material & Methods: Twenty-three obese patients undergoing gastric band surgery were recruited to a longitudinal intervention study, alongside non-obese, healthy gender and age-matched controls. Eighteen pairs of patients and controls completed all stages of the study. Peripheral blood neutrophils (PBN) were isolated by density centrifugation and a comprehensive analysis of PBN function undertaken at various stages of the patients’ bariatric surgical care pathway.
Results: Obese patients exhibited exaggerated PBN activity in response to various stimuli, characterised by higher reactive oxygen species (ROS) generation (n=18, p<0.001) and release of pro-inflammatory cytokines (n=10, p<0.05), and lower PBN extracellular trap (NET) formation (n=18, p<0.01). PBN chemotactic accuracy was also impaired prior to surgery (n=18, p<0.01). Weight loss was associated with normalised NET production and lower ROS production and cytokine release relative to healthy controls. However, chemotactic accuracy remained impaired in patients.
Conclusions: Weight loss following gastric band surgery was associated with a decrease in the pro-inflammatory activities of peripheral blood neutrophils (PBNs). A hyper-inflammatory PBN phenotype, involving excess ROS and cytokine release, reduced NET formation and chemotaxis, may lead to a reduced ability to eliminate infection, along-side inflammation-mediated tissue damage in obese individuals.
Original language | English |
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Pages (from-to) | 1611–1621 |
Number of pages | 11 |
Journal | Obesity surgery |
Volume | 28 |
Issue number | 6 |
Early online date | 14 Dec 2017 |
DOIs | |
Publication status | Published - Jun 2018 |
Keywords
- Peripheral blood neutrophil (PBN)
- Cytokines
- Neutrophil extracellular traps (NETs)
- Reactive oxygen species (ROS)
- Very Low Calorie Diet (VLCD)
- Chemotaxis
- HbA1c – glycated haemoglobin
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- 1 Finished
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Chemotaxis of Periodontal Neutrophillis:Do they display aborant behavouir (Linked to DNEN.RCFL14945)
1/05/12 → 30/06/12
Project: Research