Impact of bariatric surgical intervention on peripheral blood neutrophil (PBN) function in obesity

Helen Roberts, Melissa Grant, Naomi Hubber, Paul Super, Rishi Singhal, Iain Chapple

Research output: Contribution to journalArticlepeer-review

12 Citations (Scopus)
142 Downloads (Pure)

Abstract

Aim: To investigate the impact of weight loss following gastric band surgery on multiple measures of peripheral blood neutrophil (PBN) function.
Material & Methods: Twenty-three obese patients undergoing gastric band surgery were recruited to a longitudinal intervention study, alongside non-obese, healthy gender and age-matched controls. Eighteen pairs of patients and controls completed all stages of the study. Peripheral blood neutrophils (PBN) were isolated by density centrifugation and a comprehensive analysis of PBN function undertaken at various stages of the patients’ bariatric surgical care pathway. 
Results: Obese patients exhibited exaggerated PBN activity in response to various stimuli, characterised by higher reactive oxygen species (ROS) generation (n=18, p<0.001) and release of pro-inflammatory cytokines (n=10, p<0.05), and lower PBN extracellular trap (NET) formation (n=18, p<0.01). PBN chemotactic accuracy was also impaired prior to surgery (n=18, p<0.01). Weight loss was associated with normalised NET production and lower ROS production and cytokine release relative to healthy controls. However, chemotactic accuracy remained impaired in patients. 
Conclusions: Weight loss following gastric band surgery was associated with a decrease in the pro-inflammatory activities of peripheral blood neutrophils (PBNs). A hyper-inflammatory PBN phenotype, involving excess ROS and cytokine release, reduced NET formation and chemotaxis, may lead to a reduced ability to eliminate infection, along-side inflammation-mediated tissue damage in obese individuals.
Original languageEnglish
Pages (from-to)1611–1621
Number of pages11
JournalObesity surgery
Volume28
Issue number6
Early online date14 Dec 2017
DOIs
Publication statusPublished - Jun 2018

Keywords

  • Peripheral blood neutrophil (PBN)
  • Cytokines
  • Neutrophil extracellular traps (NETs)
  • Reactive oxygen species (ROS)
  • Very Low Calorie Diet (VLCD)
  • Chemotaxis
  • HbA1c – glycated haemoglobin

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