Immobilized WNT proteins act as a stem cell niche for tissue engineering

Molly Lowndes; Michael Rotherham; Joshua C. Price; Alicia El Haj; Shukry J. Habib

doi:10.1016/j.stemcr.2016.06.004

Immobilized WNT proteins act as a stem cell niche for tissue engineering

Molly Lowndes, Michael Rotherham, Joshua C. Price, Alicia El Haj, Shukry J. Habib^*

^*Corresponding author for this work

Chemical Engineering

Research output: Contribution to journal › Article › peer-review

12 Citations (Scopus)

155 Downloads (Pure)

Abstract

The timing, location, and level of WNT signaling are highly regulated during embryonic development and for the maintenance of adult tissues. Consequently the ability to provide a defined and directed source of WNT proteins is crucial to fully understand its role in tissue development and to mimic its activity in vitro. Here we describe a one-step immobilization technique to covalently bind WNT3A proteins as a basal surface with easy storage and long-lasting activity. We show that this platform is able to maintain adult and embryonic stem cells while also being adaptable for 3D systems. Therefore, this platform could be used for recapitulating specific stem cell niches with the goal of improving tissue engineering.

Original language	English
Pages (from-to)	126-137
Number of pages	12
Journal	Stem Cell Reports
Volume	7
Issue number	1
DOIs	https://doi.org/10.1016/j.stemcr.2016.06.004
Publication status	Published - 12 Jul 2016

ASJC Scopus subject areas

Biochemistry
Genetics
Developmental Biology
Cell Biology

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AU - Habib, Shukry J.

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AB - The timing, location, and level of WNT signaling are highly regulated during embryonic development and for the maintenance of adult tissues. Consequently the ability to provide a defined and directed source of WNT proteins is crucial to fully understand its role in tissue development and to mimic its activity in vitro. Here we describe a one-step immobilization technique to covalently bind WNT3A proteins as a basal surface with easy storage and long-lasting activity. We show that this platform is able to maintain adult and embryonic stem cells while also being adaptable for 3D systems. Therefore, this platform could be used for recapitulating specific stem cell niches with the goal of improving tissue engineering.

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Immobilized WNT proteins act as a stem cell niche for tissue engineering

Abstract

ASJC Scopus subject areas

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