Immobilized fibrinogen activates human platelets through glycoprotein VI

Pierre H Mangin, Marie Blanche Onselaer, Nicolas Receveur, Nicolas Le Lay, Alexander Hardy, Clare Wilson, Ximena Sanchez, Stephane Loyau, Arnaud Dupuis, Amir K. Babar, Jeanette L C Miller, Helen Philippou, Craig Hughes, Andrew B Herr, Robert A. S. Ariëns, Diego Mezzano, Martine Jandrot-Perrus, Christian Gachet, Steve Watson

Research output: Contribution to journalArticlepeer-review

53 Citations (Scopus)
147 Downloads (Pure)

Abstract

GPVI, a major platelet activation receptor for collagen and fibrin, is considered as a particularly promising safe antithrombotic target. In this study, we show that human GPVI is a receptor for fibrinogen. Full spreading of human platelets on fibrinogen is abolished in platelets from GPVI-deficient patients suggesting that fibrinogen activates GPVI. While mouse platelets fail to spread on fibrinogen, human-GPVI-transgenic mouse platelets show full spreading and increased Ca2+ signalling through the tyrosine kinase Syk. Direct binding of fibrinogen to human GPVI was shown by surface plasmon resonance and by increased adhesion of human GPVI-transfected Rbl-2H3 cells to fibrinogen relative to mock-transfected cells. Blockade of human GPVI with Fab 9O12 impairs platelet aggregation on preformed platelet aggregates in flowing blood independent of collagen and fibrin exposure. These results demonstrate that human GPVI binds to immobilized fibrinogen and show that this contributes to platelet spreading and platelet aggregation under flow.
Original languageEnglish
Pages (from-to)898-907
Number of pages10
JournalHaematologica
Volume103
Issue number5
Early online date22 Feb 2018
DOIs
Publication statusPublished - May 2018

Fingerprint

Dive into the research topics of 'Immobilized fibrinogen activates human platelets through glycoprotein VI'. Together they form a unique fingerprint.

Cite this