Imaging of dairy emulsions: Via a novel approach of transmission electron cryogenic microscopy using beam exposure

Kilian Daffner; Eric Hanssen; Ian Norton; Tom Mills; Lydia Ong; Sally L. Gras

doi:10.1039/d0sm00582g

Imaging of dairy emulsions: Via a novel approach of transmission electron cryogenic microscopy using beam exposure

Kilian Daffner, Eric Hanssen^*, Ian Norton, Tom Mills, Lydia Ong, Sally L. Gras

^*Corresponding author for this work

Chemical Engineering

Research output: Contribution to journal › Article › peer-review

1 Citation (Scopus)

Abstract

Emulsions containing small fat and protein particles are difficult to differentiate but low dose transmission electron cryogenic microscopy can visualize and distinguish between these droplets or particles. A controlled increase in electron dose caused the sample to bubble and visibly degrade at <25 e- Å-2 for fat globules and at >150 e- Å-2 for protein particles, allowing particle differentiation. This technique may be useful for the development of nanoemulsions, as well as nanostructured and 3D printed foods.

Original language	English
Pages (from-to)	7888-7892
Number of pages	5
Journal	Soft Matter
Volume	16
Issue number	34
DOIs	https://doi.org/10.1039/d0sm00582g
Publication status	Published - 14 Sept 2020

Bibliographical note

Funding Information:
This research was supported by the Australian Research Council’s Industrial Transformation Research Program (ITRP) funding scheme (project number IH120100005) and the Engineering and Physical Sciences Research Council [grant number EP/ N024818/1]. The authors would like to thank The Bio21 Molecular Science & Biotechnology Institute at The University of Melbourne for access to equipment. The cryo EM analysis was carried out at the Bio 21 Advanced Microscopy Facility, at The University of Melbourne. We acknowledge Unternehmensgruppe Theo Mueller for gifting the protein powders. The authors would like to thank Eddie Pelan and Fotis Spyropoulos for useful discussions and proofreading.

Publisher Copyright:
© 2020 The Royal Society of Chemistry.

Copyright:
Copyright 2020 Elsevier B.V., All rights reserved.

ASJC Scopus subject areas

General Chemistry
Condensed Matter Physics

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title = "Imaging of dairy emulsions: Via a novel approach of transmission electron cryogenic microscopy using beam exposure",

abstract = "Emulsions containing small fat and protein particles are difficult to differentiate but low dose transmission electron cryogenic microscopy can visualize and distinguish between these droplets or particles. A controlled increase in electron dose caused the sample to bubble and visibly degrade at <25 e- {\AA}-2 for fat globules and at >150 e- {\AA}-2 for protein particles, allowing particle differentiation. This technique may be useful for the development of nanoemulsions, as well as nanostructured and 3D printed foods. ",

author = "Kilian Daffner and Eric Hanssen and Ian Norton and Tom Mills and Lydia Ong and Gras, {Sally L.}",

note = "Funding Information: This research was supported by the Australian Research Council{\textquoteright}s Industrial Transformation Research Program (ITRP) funding scheme (project number IH120100005) and the Engineering and Physical Sciences Research Council [grant number EP/ N024818/1]. The authors would like to thank The Bio21 Molecular Science & Biotechnology Institute at The University of Melbourne for access to equipment. The cryo EM analysis was carried out at the Bio 21 Advanced Microscopy Facility, at The University of Melbourne. We acknowledge Unternehmensgruppe Theo Mueller for gifting the protein powders. The authors would like to thank Eddie Pelan and Fotis Spyropoulos for useful discussions and proofreading. Publisher Copyright: {\textcopyright} 2020 The Royal Society of Chemistry. Copyright: Copyright 2020 Elsevier B.V., All rights reserved.",

year = "2020",

month = sep,

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doi = "10.1039/d0sm00582g",

language = "English",

volume = "16",

pages = "7888--7892",

journal = "Soft Matter",

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T2 - Via a novel approach of transmission electron cryogenic microscopy using beam exposure

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AU - Hanssen, Eric

AU - Norton, Ian

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AU - Ong, Lydia

AU - Gras, Sally L.

N1 - Funding Information: This research was supported by the Australian Research Council’s Industrial Transformation Research Program (ITRP) funding scheme (project number IH120100005) and the Engineering and Physical Sciences Research Council [grant number EP/ N024818/1]. The authors would like to thank The Bio21 Molecular Science & Biotechnology Institute at The University of Melbourne for access to equipment. The cryo EM analysis was carried out at the Bio 21 Advanced Microscopy Facility, at The University of Melbourne. We acknowledge Unternehmensgruppe Theo Mueller for gifting the protein powders. The authors would like to thank Eddie Pelan and Fotis Spyropoulos for useful discussions and proofreading. Publisher Copyright: © 2020 The Royal Society of Chemistry. Copyright: Copyright 2020 Elsevier B.V., All rights reserved.

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Y1 - 2020/9/14

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Imaging of dairy emulsions: Via a novel approach of transmission electron cryogenic microscopy using beam exposure

Abstract

Bibliographical note

ASJC Scopus subject areas

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