Identification of the Escherichia coli cysM gene encoding O-acetylserine sulphydrylase B by cloning with mini-Mu-lac containing a plasmid replicon

A E Sirko; M Zatyka; M D Hulanicka

doi:10.1099/00221287-133-10-2719

Identification of the Escherichia coli cysM gene encoding O-acetylserine sulphydrylase B by cloning with mini-Mu-lac containing a plasmid replicon

A E Sirko, M Zatyka, M D Hulanicka

Cancer and Genomic Sciences

Research output: Contribution to journal › Article › peer-review

17 Citations (Scopus)

Abstract

A region located at around 52' on the Escherichia coli chromosome was cloned by use of mini-Mu-lac containing a plasmid replicon and recloned into pBR322. Enzyme assays on transformants carrying the cloned fragments indicated the presence in the latter of the cysA and cysM genes coding for sulphate permease and O-acetylserine sulphydrylase B, respectively.

Original language	English
Pages (from-to)	2719-25
Number of pages	7
Journal	Journal of general microbiology
Volume	133
Issue number	10
DOIs	https://doi.org/10.1099/00221287-133-10-2719
Publication status	Published - Oct 1987

Keywords

Cloning, Molecular
Cysteine/genetics
Cysteine Synthase/genetics
Escherichia coli/enzymology
Genes, Bacterial
Lyases/genetics
Plasmids
Replicon

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10.1099/00221287-133-10-2719

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abstract = "A region located at around 52' on the Escherichia coli chromosome was cloned by use of mini-Mu-lac containing a plasmid replicon and recloned into pBR322. Enzyme assays on transformants carrying the cloned fragments indicated the presence in the latter of the cysA and cysM genes coding for sulphate permease and O-acetylserine sulphydrylase B, respectively.",

keywords = "Cloning, Molecular, Cysteine/genetics, Cysteine Synthase/genetics, Escherichia coli/enzymology, Genes, Bacterial, Lyases/genetics, Plasmids, Replicon",

author = "Sirko, {A E} and M Zatyka and Hulanicka, {M D}",

year = "1987",

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AU - Zatyka, M

AU - Hulanicka, M D

PY - 1987/10

Y1 - 1987/10

N2 - A region located at around 52' on the Escherichia coli chromosome was cloned by use of mini-Mu-lac containing a plasmid replicon and recloned into pBR322. Enzyme assays on transformants carrying the cloned fragments indicated the presence in the latter of the cysA and cysM genes coding for sulphate permease and O-acetylserine sulphydrylase B, respectively.

AB - A region located at around 52' on the Escherichia coli chromosome was cloned by use of mini-Mu-lac containing a plasmid replicon and recloned into pBR322. Enzyme assays on transformants carrying the cloned fragments indicated the presence in the latter of the cysA and cysM genes coding for sulphate permease and O-acetylserine sulphydrylase B, respectively.

KW - Cloning, Molecular

KW - Cysteine/genetics

KW - Cysteine Synthase/genetics

KW - Escherichia coli/enzymology

KW - Genes, Bacterial

KW - Lyases/genetics

KW - Plasmids

KW - Replicon

U2 - 10.1099/00221287-133-10-2719

DO - 10.1099/00221287-133-10-2719

M3 - Article

C2 - 3329675

SN - 0022-1287

VL - 133

SP - 2719

EP - 2725

JO - Journal of general microbiology

JF - Journal of general microbiology

IS - 10

ER -

Identification of the Escherichia coli cysM gene encoding O-acetylserine sulphydrylase B by cloning with mini-Mu-lac containing a plasmid replicon

Abstract

Keywords

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