A region located at around 52' on the Escherichia coli chromosome was cloned by use of mini-Mu-lac containing a plasmid replicon and recloned into pBR322. Enzyme assays on transformants carrying the cloned fragments indicated the presence in the latter of the cysA and cysM genes coding for sulphate permease and O-acetylserine sulphydrylase B, respectively.
|Number of pages||7|
|Journal||Journal of general microbiology|
|Publication status||Published - Oct 1987|
- Cloning, Molecular
- Cysteine Synthase/genetics
- Escherichia coli/enzymology
- Genes, Bacterial