Identification of a genetic determinant in clinical Enterococcus faecium strains that contributes to intestinal colonization during antibiotic treatment

Xinglin Zhang; Janetta Top; Mark de Been; Damien Bierschenk; Malbert Rogers; Masja Leendertse; Marc J M Bonten; Tom van der Poll; Rob J L Willems; Willem van Schaik

doi:10.1093/infdis/jit076

Identification of a genetic determinant in clinical Enterococcus faecium strains that contributes to intestinal colonization during antibiotic treatment

Xinglin Zhang, Janetta Top, Mark de Been, Damien Bierschenk, Malbert Rogers, Masja Leendertse, Marc J M Bonten, Tom van der Poll, Rob J L Willems, Willem van Schaik

Microbes, Infections and Microbiomes

Research output: Contribution to journal › Article › peer-review

54 Citations (Scopus)

Abstract

Intestinal colonization by antibiotic-resistant Enterococcus faecium is the first step in a process that can lead to infections in hospitalized patients. By comparative genome analysis and subsequent polymerase chain reaction screening, we identified a locus that encodes a putative phosphotransferase system (PTS). The PTS locus was widespread in isolates from hospital outbreaks of infection (84.2%) and nonoutbreak clinical infections (66.0%) but absent from human commensal isolates. Deletion of pstD, which is predicted to encode the enzyme IID subunit of this PTS, significantly impaired the ability of E. faecium to colonize the murine intestinal tract during antibiotic treatment. This is the first description of a determinant that contributes to intestinal colonization in clinical E. faecium strains.

Original language	English
Pages (from-to)	1780-6
Number of pages	7
Journal	The Journal of Infectious Diseases
Volume	207
Issue number	11
DOIs	https://doi.org/10.1093/infdis/jit076
Publication status	Published - 1 Jun 2013

Keywords

Animals
Anti-Bacterial Agents
Carrier State
Disease Models, Animal
Enterococcus faecium
Gastrointestinal Tract
Gene Deletion
Gram-Positive Bacterial Infections
Humans
Male
Mice
Mice, Inbred BALB C
Phosphotransferases
Virulence Factors
Journal Article
Research Support, Non-U.S. Gov't

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10.1093/infdis/jit076

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Zhang, X., Top, J., de Been, M., Bierschenk, D., Rogers, M., Leendertse, M., Bonten, M. J. M., van der Poll, T., Willems, R. J. L., & van Schaik, W. (2013). Identification of a genetic determinant in clinical Enterococcus faecium strains that contributes to intestinal colonization during antibiotic treatment. The Journal of Infectious Diseases, 207(11), 1780-6. https://doi.org/10.1093/infdis/jit076

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title = "Identification of a genetic determinant in clinical Enterococcus faecium strains that contributes to intestinal colonization during antibiotic treatment",

abstract = "Intestinal colonization by antibiotic-resistant Enterococcus faecium is the first step in a process that can lead to infections in hospitalized patients. By comparative genome analysis and subsequent polymerase chain reaction screening, we identified a locus that encodes a putative phosphotransferase system (PTS). The PTS locus was widespread in isolates from hospital outbreaks of infection (84.2%) and nonoutbreak clinical infections (66.0%) but absent from human commensal isolates. Deletion of pstD, which is predicted to encode the enzyme IID subunit of this PTS, significantly impaired the ability of E. faecium to colonize the murine intestinal tract during antibiotic treatment. This is the first description of a determinant that contributes to intestinal colonization in clinical E. faecium strains.",

keywords = "Animals, Anti-Bacterial Agents, Carrier State, Disease Models, Animal, Enterococcus faecium, Gastrointestinal Tract, Gene Deletion, Gram-Positive Bacterial Infections, Humans, Male, Mice, Mice, Inbred BALB C, Phosphotransferases, Virulence Factors, Journal Article, Research Support, Non-U.S. Gov't",

author = "Xinglin Zhang and Janetta Top and {de Been}, Mark and Damien Bierschenk and Malbert Rogers and Masja Leendertse and Bonten, {Marc J M} and {van der Poll}, Tom and Willems, {Rob J L} and {van Schaik}, Willem",

year = "2013",

month = jun,

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doi = "10.1093/infdis/jit076",

language = "English",

volume = "207",

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journal = "The Journal of Infectious Diseases",

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publisher = "Oxford University Press",

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Zhang, X, Top, J, de Been, M, Bierschenk, D, Rogers, M, Leendertse, M, Bonten, MJM, van der Poll, T, Willems, RJL & van Schaik, W 2013, 'Identification of a genetic determinant in clinical Enterococcus faecium strains that contributes to intestinal colonization during antibiotic treatment', The Journal of Infectious Diseases, vol. 207, no. 11, pp. 1780-6. https://doi.org/10.1093/infdis/jit076

TY - JOUR

T1 - Identification of a genetic determinant in clinical Enterococcus faecium strains that contributes to intestinal colonization during antibiotic treatment

AU - Zhang, Xinglin

AU - Top, Janetta

AU - de Been, Mark

AU - Bierschenk, Damien

AU - Rogers, Malbert

AU - Leendertse, Masja

AU - Bonten, Marc J M

AU - van der Poll, Tom

AU - Willems, Rob J L

AU - van Schaik, Willem

PY - 2013/6/1

Y1 - 2013/6/1

N2 - Intestinal colonization by antibiotic-resistant Enterococcus faecium is the first step in a process that can lead to infections in hospitalized patients. By comparative genome analysis and subsequent polymerase chain reaction screening, we identified a locus that encodes a putative phosphotransferase system (PTS). The PTS locus was widespread in isolates from hospital outbreaks of infection (84.2%) and nonoutbreak clinical infections (66.0%) but absent from human commensal isolates. Deletion of pstD, which is predicted to encode the enzyme IID subunit of this PTS, significantly impaired the ability of E. faecium to colonize the murine intestinal tract during antibiotic treatment. This is the first description of a determinant that contributes to intestinal colonization in clinical E. faecium strains.

AB - Intestinal colonization by antibiotic-resistant Enterococcus faecium is the first step in a process that can lead to infections in hospitalized patients. By comparative genome analysis and subsequent polymerase chain reaction screening, we identified a locus that encodes a putative phosphotransferase system (PTS). The PTS locus was widespread in isolates from hospital outbreaks of infection (84.2%) and nonoutbreak clinical infections (66.0%) but absent from human commensal isolates. Deletion of pstD, which is predicted to encode the enzyme IID subunit of this PTS, significantly impaired the ability of E. faecium to colonize the murine intestinal tract during antibiotic treatment. This is the first description of a determinant that contributes to intestinal colonization in clinical E. faecium strains.

KW - Animals

KW - Anti-Bacterial Agents

KW - Carrier State

KW - Disease Models, Animal

KW - Enterococcus faecium

KW - Gastrointestinal Tract

KW - Gene Deletion

KW - Gram-Positive Bacterial Infections

KW - Humans

KW - Male

KW - Mice

KW - Mice, Inbred BALB C

KW - Phosphotransferases

KW - Virulence Factors

KW - Journal Article

KW - Research Support, Non-U.S. Gov't

U2 - 10.1093/infdis/jit076

DO - 10.1093/infdis/jit076

M3 - Article

C2 - 23447698

SN - 0022-1899

VL - 207

SP - 1780

EP - 1786

JO - The Journal of Infectious Diseases

JF - The Journal of Infectious Diseases

IS - 11

ER -

Identification of a genetic determinant in clinical Enterococcus faecium strains that contributes to intestinal colonization during antibiotic treatment

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Keywords

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