Hydrogel-based Sensing Technology for Quantitative Measurements of Low Concentrations of Proteins in the Presence of Interferents

Khalid Haliru; Ruchi Gupta

doi:10.5162/EUROSENSORSXXXVI/OT6.7

Hydrogel-based Sensing Technology for Quantitative Measurements of Low Concentrations of Proteins in the Presence of Interferents

Khalid Haliru, Ruchi Gupta^*

^*Corresponding author for this work

Chemistry

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution

Abstract

We report a hydrogel-based sensing technology for the measurement of proteins with a limit of detection of 0.003 ppm or 53 pM for an exemplar analyte, streptavidin, while being less laborious than the state-of-the-art method, enzyme-linked immunosorbent assay. Equally, we showed that the presence of high molecular weight interferents such as mucin at 100- and 1000-times higher concentrations than the exemplar analyte did not influence protein measurement.

Original language	English
Title of host publication	Association for Sensors Measurement
Publisher	Association for Sensors and Measurement (AMA)
Pages	151 - 152
ISBN (Electronic)	9783910600034
DOIs	https://doi.org/10.5162/EUROSENSORSXXXVI/OT6.7
Publication status	Published - 4 Sept 2024
Event	EUROSENSORS XXXVI - Debrecen, Hungary Duration: 1 Sept 2024 → 4 Sept 2024 https://akcongress.com/eurosensors/

Conference

Conference	EUROSENSORS XXXVI
Country/Territory	Hungary
City	Debrecen
Period	1/09/24 → 4/09/24
Internet address	https://akcongress.com/eurosensors/

Access to Document

10.5162/EUROSENSORSXXXVI/OT6.7Licence: None: All rights reserved

Cite this

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title = "Hydrogel-based Sensing Technology for Quantitative Measurements of Low Concentrations of Proteins in the Presence of Interferents",

abstract = "We report a hydrogel-based sensing technology for the measurement of proteins with a limit of detection of 0.003 ppm or 53 pM for an exemplar analyte, streptavidin, while being less laborious than the state-of-the-art method, enzyme-linked immunosorbent assay. Equally, we showed that the presence of high molecular weight interferents such as mucin at 100- and 1000-times higher concentrations than the exemplar analyte did not influence protein measurement.",

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AU - Gupta, Ruchi

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N2 - We report a hydrogel-based sensing technology for the measurement of proteins with a limit of detection of 0.003 ppm or 53 pM for an exemplar analyte, streptavidin, while being less laborious than the state-of-the-art method, enzyme-linked immunosorbent assay. Equally, we showed that the presence of high molecular weight interferents such as mucin at 100- and 1000-times higher concentrations than the exemplar analyte did not influence protein measurement.

AB - We report a hydrogel-based sensing technology for the measurement of proteins with a limit of detection of 0.003 ppm or 53 pM for an exemplar analyte, streptavidin, while being less laborious than the state-of-the-art method, enzyme-linked immunosorbent assay. Equally, we showed that the presence of high molecular weight interferents such as mucin at 100- and 1000-times higher concentrations than the exemplar analyte did not influence protein measurement.

U2 - 10.5162/EUROSENSORSXXXVI/OT6.7

DO - 10.5162/EUROSENSORSXXXVI/OT6.7

M3 - Conference contribution

SP - 151

EP - 152

BT - Association for Sensors Measurement

PB - Association for Sensors and Measurement (AMA)

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