Human Papillomavirus E6/E7 mRNA detection by in situ hybridization in oral cavity squamous cell carcinoma

Priscila Marinho Abreu; Isabella Bittencourt Valle; Thabata Coeli Dias Damasceno; Anna Clara Gregório Có; Paola Fernandes Pansini; José Roberto Vasconcelos Podestá; Evandro Duccini Souza; Ricardo Mai Rocha; Maria Paula Curado; Hisham Mehanna; Sandra Ventorin von Zeidler

doi:10.1016/j.archoralbio.2020.104746

Human Papillomavirus E6/E7 mRNA detection by in situ hybridization in oral cavity squamous cell carcinoma

Priscila Marinho Abreu
, Isabella Bittencourt Valle
, Thabata Coeli Dias Damasceno
, Anna Clara Gregório Có
, Paola Fernandes Pansini
, José Roberto Vasconcelos Podestá
, Evandro Duccini Souza
, Ricardo Mai Rocha
, Maria Paula Curado
, Hisham Mehanna
, Sandra Ventorin von Zeidler^*

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

3 Citations (Scopus)

Abstract

Objective: The aim of this study was to evaluate the application of in situ hybridization using E6/E7 mRNA probes to identify the frequency of high-risk HPV transcriptionally active and the use of HPV status as a prognostic biomarker in oral cavity squamous cell carcinoma (OCSCC).

Methods: Ninety-nine OCSCC samples were evaluated from Hospital Santa Rita de Cassia, Hospital Universitário Cassiano Antônio de Moraes and University Hospitals Coventry and Warwickshire NHS Trust. After tissue microarray construction, the slides were submitted to an in situ hybridization detection method for HPV E6/E7 mRNA. HPV status was designated a binary classification. Multiple logistic regression examined the association of HPV with clinical features and other risk factors, using SPSS® software. For all hypothesis tests, a significance level of p ≤ 0.05 was considered.

Results: HPV frequency in oral squamous cell carcinoma was 8%. There was no association between HPV and clinical variables and between the main prognostic features and known risk factors. There was no difference in the prevalence of HPV for oral cavity squamous cell carcinoma by geography (Brazil vs UK).

Conclusions: A low frequency of E6/E7 mRNA by RNA in situ hybridization was found in oral cavity squamous cell carcinoma, which supports the evidence that HPV-driven cancer of the oral cavity is uncommon.

Original language	English
Article number	104746
Journal	Archives of Oral Biology
Volume	116
Early online date	7 May 2020
DOIs	https://doi.org/10.1016/j.archoralbio.2020.104746
Publication status	Published - Aug 2020

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

SDG 3 Good Health and Well-being

Keywords

Head and neck cancer
High risk human papillomavirus
HPV-driven cancer
RNA in situ hybridization

ASJC Scopus subject areas

Otorhinolaryngology
General Dentistry
Cell Biology

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10.1016/j.archoralbio.2020.104746

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Abreu, P. M., Valle, I. B., Damasceno, T. C. D., Có, A. C. G., Pansini, P. F., Podestá, J. R. V., Souza, E. D., Rocha, R. M., Curado, M. P., Mehanna, H., & von Zeidler, S. V. (2020). Human Papillomavirus E6/E7 mRNA detection by in situ hybridization in oral cavity squamous cell carcinoma. Archives of Oral Biology, 116, Article 104746. https://doi.org/10.1016/j.archoralbio.2020.104746

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title = "Human Papillomavirus E6/E7 mRNA detection by in situ hybridization in oral cavity squamous cell carcinoma",

abstract = "Objective: The aim of this study was to evaluate the application of in situ hybridization using E6/E7 mRNA probes to identify the frequency of high-risk HPV transcriptionally active and the use of HPV status as a prognostic biomarker in oral cavity squamous cell carcinoma (OCSCC). Methods: Ninety-nine OCSCC samples were evaluated from Hospital Santa Rita de Cassia, Hospital Universit{\'a}rio Cassiano Ant{\^o}nio de Moraes and University Hospitals Coventry and Warwickshire NHS Trust. After tissue microarray construction, the slides were submitted to an in situ hybridization detection method for HPV E6/E7 mRNA. HPV status was designated a binary classification. Multiple logistic regression examined the association of HPV with clinical features and other risk factors, using SPSS{\textregistered} software. For all hypothesis tests, a significance level of p ≤ 0.05 was considered. Results: HPV frequency in oral squamous cell carcinoma was 8\%. There was no association between HPV and clinical variables and between the main prognostic features and known risk factors. There was no difference in the prevalence of HPV for oral cavity squamous cell carcinoma by geography (Brazil vs UK). Conclusions: A low frequency of E6/E7 mRNA by RNA in situ hybridization was found in oral cavity squamous cell carcinoma, which supports the evidence that HPV-driven cancer of the oral cavity is uncommon.",

keywords = "Head and neck cancer, High risk human papillomavirus, HPV-driven cancer, RNA in situ hybridization",

author = "Abreu, \{Priscila Marinho\} and Valle, \{Isabella Bittencourt\} and Damasceno, \{Thabata Coeli Dias\} and C{\'o}, \{Anna Clara Greg{\'o}rio\} and Pansini, \{Paola Fernandes\} and Podest{\'a}, \{Jos{\'e} Roberto Vasconcelos\} and Souza, \{Evandro Duccini\} and Rocha, \{Ricardo Mai\} and Curado, \{Maria Paula\} and Hisham Mehanna and \{von Zeidler\}, \{Sandra Ventorin\}",

year = "2020",

month = aug,

doi = "10.1016/j.archoralbio.2020.104746",

language = "English",

volume = "116",

journal = "Archives of Oral Biology",

issn = "0003-9969",

publisher = "Elsevier",

}

TY - JOUR

T1 - Human Papillomavirus E6/E7 mRNA detection by in situ hybridization in oral cavity squamous cell carcinoma

AU - Abreu, Priscila Marinho

AU - Valle, Isabella Bittencourt

AU - Damasceno, Thabata Coeli Dias

AU - Có, Anna Clara Gregório

AU - Pansini, Paola Fernandes

AU - Podestá, José Roberto Vasconcelos

AU - Souza, Evandro Duccini

AU - Rocha, Ricardo Mai

AU - Curado, Maria Paula

AU - Mehanna, Hisham

AU - von Zeidler, Sandra Ventorin

PY - 2020/8

Y1 - 2020/8

N2 - Objective: The aim of this study was to evaluate the application of in situ hybridization using E6/E7 mRNA probes to identify the frequency of high-risk HPV transcriptionally active and the use of HPV status as a prognostic biomarker in oral cavity squamous cell carcinoma (OCSCC). Methods: Ninety-nine OCSCC samples were evaluated from Hospital Santa Rita de Cassia, Hospital Universitário Cassiano Antônio de Moraes and University Hospitals Coventry and Warwickshire NHS Trust. After tissue microarray construction, the slides were submitted to an in situ hybridization detection method for HPV E6/E7 mRNA. HPV status was designated a binary classification. Multiple logistic regression examined the association of HPV with clinical features and other risk factors, using SPSS® software. For all hypothesis tests, a significance level of p ≤ 0.05 was considered. Results: HPV frequency in oral squamous cell carcinoma was 8%. There was no association between HPV and clinical variables and between the main prognostic features and known risk factors. There was no difference in the prevalence of HPV for oral cavity squamous cell carcinoma by geography (Brazil vs UK). Conclusions: A low frequency of E6/E7 mRNA by RNA in situ hybridization was found in oral cavity squamous cell carcinoma, which supports the evidence that HPV-driven cancer of the oral cavity is uncommon.

AB - Objective: The aim of this study was to evaluate the application of in situ hybridization using E6/E7 mRNA probes to identify the frequency of high-risk HPV transcriptionally active and the use of HPV status as a prognostic biomarker in oral cavity squamous cell carcinoma (OCSCC). Methods: Ninety-nine OCSCC samples were evaluated from Hospital Santa Rita de Cassia, Hospital Universitário Cassiano Antônio de Moraes and University Hospitals Coventry and Warwickshire NHS Trust. After tissue microarray construction, the slides were submitted to an in situ hybridization detection method for HPV E6/E7 mRNA. HPV status was designated a binary classification. Multiple logistic regression examined the association of HPV with clinical features and other risk factors, using SPSS® software. For all hypothesis tests, a significance level of p ≤ 0.05 was considered. Results: HPV frequency in oral squamous cell carcinoma was 8%. There was no association between HPV and clinical variables and between the main prognostic features and known risk factors. There was no difference in the prevalence of HPV for oral cavity squamous cell carcinoma by geography (Brazil vs UK). Conclusions: A low frequency of E6/E7 mRNA by RNA in situ hybridization was found in oral cavity squamous cell carcinoma, which supports the evidence that HPV-driven cancer of the oral cavity is uncommon.

KW - Head and neck cancer

KW - High risk human papillomavirus

KW - HPV-driven cancer

KW - RNA in situ hybridization

UR - https://www.scopus.com/pages/publications/85085160567

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DO - 10.1016/j.archoralbio.2020.104746

M3 - Article

AN - SCOPUS:85085160567

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VL - 116

JO - Archives of Oral Biology

JF - Archives of Oral Biology

M1 - 104746

ER -

Human Papillomavirus E6/E7 mRNA detection by in situ hybridization in oral cavity squamous cell carcinoma

Abstract

UN SDGs

Keywords

ASJC Scopus subject areas

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