Human induced pluripotent stem cells are capable of B-cell lymphopoiesis

Lee Carpenter; Ram Malladi; Cheng-Tao Yang; Anna French; Katherine J Pilkington; Richard W Forsey; Jackie Sloane-Stanley; Kathryn M Silk; Timothy J Davies; Paul J Fairchild; Tariq Enver; Suzanne M Watt

doi:10.1182/blood-2010-08-299941

Human induced pluripotent stem cells are capable of B-cell lymphopoiesis

Lee Carpenter, Ram Malladi, Cheng-Tao Yang, Anna French, Katherine J Pilkington, Richard W Forsey, Jackie Sloane-Stanley, Kathryn M Silk, Timothy J Davies, Paul J Fairchild, Tariq Enver, Suzanne M Watt

Cancer and Genomic Sciences

Research output: Contribution to journal › Article › peer-review

55 Citations (Scopus)

Abstract

Induced pluripotent stem (iPS) cells offer a unique potential for understanding the molecular basis of disease and development. Here we have generated several human iPS cell lines, and we describe their pluripotent phenotype and ability to differentiate into erythroid cells, monocytes, and endothelial cells. More significantly, however, when these iPS cells were differentiated under conditions that promote lympho-hematopoiesis from human embryonic stem cells, we observed the formation of pre-B cells. These cells were CD45(+)CD19(+)CD10(+) and were positive for transcripts Pax5, IL7αR, λ-like, and VpreB receptor. Although they were negative for surface IgM and CD5 expression, iPS-derived CD45(+)CD19(+) cells also exhibited multiple genomic D-J(H) rearrangements, which supports a pre-B-cell identity. We therefore have been able to demonstrate, for the first time, that human iPS cells are able to undergo hematopoiesis that contributes to the B-cell lymphoid lineage.

Original language	English
Pages (from-to)	4008-11
Number of pages	4
Journal	Blood
Volume	117
Issue number	15
DOIs	https://doi.org/10.1182/blood-2010-08-299941
Publication status	Published - 14 Apr 2011

Keywords

Lymphopoiesis
Pluripotent Stem Cells
Cell Lineage
Humans
Neprilysin
B-Lymphocytes
Immunoglobulin Light Chains, Surrogate
Receptors, Interleukin-7
Antigens, CD45
Antigens, CD19
B-Cell-Specific Activator Protein
Precursor Cells, B-Lymphoid
Adult
Immunophenotyping
Cell Line

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

Access to Document

10.1182/blood-2010-08-299941

Cite this

@article{1bfb3d4917784e5c8e421e81158b80f5,

title = "Human induced pluripotent stem cells are capable of B-cell lymphopoiesis",

abstract = "Induced pluripotent stem (iPS) cells offer a unique potential for understanding the molecular basis of disease and development. Here we have generated several human iPS cell lines, and we describe their pluripotent phenotype and ability to differentiate into erythroid cells, monocytes, and endothelial cells. More significantly, however, when these iPS cells were differentiated under conditions that promote lympho-hematopoiesis from human embryonic stem cells, we observed the formation of pre-B cells. These cells were CD45(+)CD19(+)CD10(+) and were positive for transcripts Pax5, IL7αR, λ-like, and VpreB receptor. Although they were negative for surface IgM and CD5 expression, iPS-derived CD45(+)CD19(+) cells also exhibited multiple genomic D-J(H) rearrangements, which supports a pre-B-cell identity. We therefore have been able to demonstrate, for the first time, that human iPS cells are able to undergo hematopoiesis that contributes to the B-cell lymphoid lineage.",

keywords = "Lymphopoiesis, Pluripotent Stem Cells, Cell Lineage, Humans, Neprilysin, B-Lymphocytes, Immunoglobulin Light Chains, Surrogate, Receptors, Interleukin-7, Antigens, CD45, Antigens, CD19, B-Cell-Specific Activator Protein, Precursor Cells, B-Lymphoid, Adult, Immunophenotyping, Cell Line",

author = "Lee Carpenter and Ram Malladi and Cheng-Tao Yang and Anna French and Pilkington, {Katherine J} and Forsey, {Richard W} and Jackie Sloane-Stanley and Silk, {Kathryn M} and Davies, {Timothy J} and Fairchild, {Paul J} and Tariq Enver and Watt, {Suzanne M}",

year = "2011",

month = apr,

day = "14",

doi = "10.1182/blood-2010-08-299941",

language = "English",

volume = "117",

pages = "4008--11",

journal = "Blood",

issn = "1528-0020",

publisher = "American Society of Hematology",

number = "15",

}

TY - JOUR

T1 - Human induced pluripotent stem cells are capable of B-cell lymphopoiesis

AU - Carpenter, Lee

AU - Malladi, Ram

AU - Yang, Cheng-Tao

AU - French, Anna

AU - Pilkington, Katherine J

AU - Forsey, Richard W

AU - Sloane-Stanley, Jackie

AU - Silk, Kathryn M

AU - Davies, Timothy J

AU - Fairchild, Paul J

AU - Enver, Tariq

AU - Watt, Suzanne M

PY - 2011/4/14

Y1 - 2011/4/14

N2 - Induced pluripotent stem (iPS) cells offer a unique potential for understanding the molecular basis of disease and development. Here we have generated several human iPS cell lines, and we describe their pluripotent phenotype and ability to differentiate into erythroid cells, monocytes, and endothelial cells. More significantly, however, when these iPS cells were differentiated under conditions that promote lympho-hematopoiesis from human embryonic stem cells, we observed the formation of pre-B cells. These cells were CD45(+)CD19(+)CD10(+) and were positive for transcripts Pax5, IL7αR, λ-like, and VpreB receptor. Although they were negative for surface IgM and CD5 expression, iPS-derived CD45(+)CD19(+) cells also exhibited multiple genomic D-J(H) rearrangements, which supports a pre-B-cell identity. We therefore have been able to demonstrate, for the first time, that human iPS cells are able to undergo hematopoiesis that contributes to the B-cell lymphoid lineage.

AB - Induced pluripotent stem (iPS) cells offer a unique potential for understanding the molecular basis of disease and development. Here we have generated several human iPS cell lines, and we describe their pluripotent phenotype and ability to differentiate into erythroid cells, monocytes, and endothelial cells. More significantly, however, when these iPS cells were differentiated under conditions that promote lympho-hematopoiesis from human embryonic stem cells, we observed the formation of pre-B cells. These cells were CD45(+)CD19(+)CD10(+) and were positive for transcripts Pax5, IL7αR, λ-like, and VpreB receptor. Although they were negative for surface IgM and CD5 expression, iPS-derived CD45(+)CD19(+) cells also exhibited multiple genomic D-J(H) rearrangements, which supports a pre-B-cell identity. We therefore have been able to demonstrate, for the first time, that human iPS cells are able to undergo hematopoiesis that contributes to the B-cell lymphoid lineage.

KW - Lymphopoiesis

KW - Pluripotent Stem Cells

KW - Cell Lineage

KW - Humans

KW - Neprilysin

KW - B-Lymphocytes

KW - Immunoglobulin Light Chains, Surrogate

KW - Receptors, Interleukin-7

KW - Antigens, CD45

KW - Antigens, CD19

KW - B-Cell-Specific Activator Protein

KW - Precursor Cells, B-Lymphoid

KW - Adult

KW - Immunophenotyping

KW - Cell Line

U2 - 10.1182/blood-2010-08-299941

DO - 10.1182/blood-2010-08-299941

M3 - Article

C2 - 21343609

SN - 1528-0020

VL - 117

SP - 4008

EP - 4011

JO - Blood

JF - Blood

IS - 15

ER -

Human induced pluripotent stem cells are capable of B-cell lymphopoiesis

Abstract

Keywords

UN SDGs

Access to Document

Fingerprint

Cite this