Human herpesvirus 8 infection DNA positivity is associated with low insulin secretion: A case-control study in a sub-Saharan African population with diabetes

Eric Lontchi-Yimagou; Jérôme Legoff; Jean-Louis Nguewa; Philippe Boudou; Eric V Balti; Jean J Noubiap; Vicky Kamwa; Barbara Atogho-Tiedeu; Marcel Azabji-Kenfack; Eric N Djahmeni; Martine Etoa; Gaelle Lemdjo; Vanessa Balla; Mesmin Y Dehayem; Fabienne Foufelle; Jean-Claude Mbanya; Jean-Francois Gautier; Eugene Sobngwi; Vicky Kamwa

doi:10.1111/1753-0407.12777

Human herpesvirus 8 infection DNA positivity is associated with low insulin secretion: A case-control study in a sub-Saharan African population with diabetes

Eric Lontchi-Yimagou, Jérôme Legoff, Jean-Louis Nguewa, Philippe Boudou, Eric V Balti, Jean J Noubiap, Vicky Kamwa, Barbara Atogho-Tiedeu, Marcel Azabji-Kenfack, Eric N Djahmeni, Martine Etoa, Gaelle Lemdjo, Vanessa Balla, Mesmin Y Dehayem, Fabienne Foufelle, Jean-Claude Mbanya, Jean-Francois Gautier, Eugene Sobngwi, Vicky Kamwa

Inflammation and Ageing

Research output: Contribution to journal › Article › peer-review

Abstract

BACKGROUND: Viruses have been considered potential triggers for the development of diabetes. This study assessed insulin secretion and insulin sensitivity in human herpesvirus 8 (HHV8)-infected and uninfected sub-Saharan African people with diabetes.

METHODS: In all, 173 people with non-autoimmune diabetes were enrolled consecutively: 124 with type 2 diabetes mellitus (T2DM) and 49 with ketosis-prone diabetes (KPD) admitted in hyperglycemic crisis. Those with KPD were further subdivided into those with new-onset ketotic-phase KPD (n = 34) or non-ketotic phase KPD (n = 15). All participants were screened for HHV8-specific antibodies and genomic DNA. Blood samples were collected for analysis of fasting glucose, HbA1c, lipid profile, and C-peptide, with insulin resistance and secretion estimated by homeostasis model assessment.

RESULTS: Among the 173 diabetic participants, 88 (50.9%) were positive for HHV8 antibodies (Ac-HHV8+), including 15 (8.7%) positive for HHV8 DNA (DNA-HHV8+). The seroprevalence of HHV8 was similar between T2DM (55.6%) and KPD (61.2%) subjects. Of those with and without ketotic-phase KPD, 35.3% and 46.7% were Ac-HHV8+, respectively. Body mass index was significantly in lower DNA-HHV8+ than DNA-HHV8- subjects. Low-density lipoprotein and total cholesterol were significantly higher, but C-peptide and homeostatic model assessment of β-cell function (HOMA-β) were significantly lower in DNA-HHV8+ than DNA-HHV8- participants. After excluding DNA-HHV8+ participants, triglyceride concentrations were significantly higher in Ac-HHV8+ (n = 73) than Ac-HHV8- (n = 85) subjects. In contrast, HOMA-β was significantly higher among Ac-HHV8+ than Ac-HHV8- participants.

CONCLUSIONS: In the present study, HHV8 DNA positivity was associated with low insulin secretion in this sub-Saharan African diabetes population.

Original language	English
Pages (from-to)	866-873
Number of pages	8
Journal	Journal of Diabetes
Volume	10
Issue number	11
DOIs	https://doi.org/10.1111/1753-0407.12777
Publication status	Published - Nov 2018

Bibliographical note

Keywords

Adult
Biomarkers/blood
Cameroon/epidemiology
Case-Control Studies
DNA, Viral/genetics
Diabetes Mellitus/blood
Female
Herpesviridae Infections/blood
Herpesvirus 8, Human/genetics
Host-Pathogen Interactions
Humans
Insulin/blood
Male
Middle Aged
Risk Factors
Secretory Pathway
Viral Load

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.1111/1753-0407.12777

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Lontchi-Yimagou, E., Legoff, J., Nguewa, J.-L., Boudou, P., Balti, E. V., Noubiap, J. J., Kamwa, V., Atogho-Tiedeu, B., Azabji-Kenfack, M., Djahmeni, E. N., Etoa, M., Lemdjo, G., Balla, V., Dehayem, M. Y., Foufelle, F., Mbanya, J.-C., Gautier, J.-F., Sobngwi, E., & Kamwa, V. (2018). Human herpesvirus 8 infection DNA positivity is associated with low insulin secretion: A case-control study in a sub-Saharan African population with diabetes. Journal of Diabetes, 10(11), 866-873. https://doi.org/10.1111/1753-0407.12777

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title = "Human herpesvirus 8 infection DNA positivity is associated with low insulin secretion: A case-control study in a sub-Saharan African population with diabetes",

abstract = "BACKGROUND: Viruses have been considered potential triggers for the development of diabetes. This study assessed insulin secretion and insulin sensitivity in human herpesvirus 8 (HHV8)-infected and uninfected sub-Saharan African people with diabetes.METHODS: In all, 173 people with non-autoimmune diabetes were enrolled consecutively: 124 with type 2 diabetes mellitus (T2DM) and 49 with ketosis-prone diabetes (KPD) admitted in hyperglycemic crisis. Those with KPD were further subdivided into those with new-onset ketotic-phase KPD (n = 34) or non-ketotic phase KPD (n = 15). All participants were screened for HHV8-specific antibodies and genomic DNA. Blood samples were collected for analysis of fasting glucose, HbA1c, lipid profile, and C-peptide, with insulin resistance and secretion estimated by homeostasis model assessment.RESULTS: Among the 173 diabetic participants, 88 (50.9%) were positive for HHV8 antibodies (Ac-HHV8+), including 15 (8.7%) positive for HHV8 DNA (DNA-HHV8+). The seroprevalence of HHV8 was similar between T2DM (55.6%) and KPD (61.2%) subjects. Of those with and without ketotic-phase KPD, 35.3% and 46.7% were Ac-HHV8+, respectively. Body mass index was significantly in lower DNA-HHV8+ than DNA-HHV8- subjects. Low-density lipoprotein and total cholesterol were significantly higher, but C-peptide and homeostatic model assessment of β-cell function (HOMA-β) were significantly lower in DNA-HHV8+ than DNA-HHV8- participants. After excluding DNA-HHV8+ participants, triglyceride concentrations were significantly higher in Ac-HHV8+ (n = 73) than Ac-HHV8- (n = 85) subjects. In contrast, HOMA-β was significantly higher among Ac-HHV8+ than Ac-HHV8- participants.CONCLUSIONS: In the present study, HHV8 DNA positivity was associated with low insulin secretion in this sub-Saharan African diabetes population.",

keywords = "Adult, Biomarkers/blood, Cameroon/epidemiology, Case-Control Studies, DNA, Viral/genetics, Diabetes Mellitus/blood, Female, Herpesviridae Infections/blood, Herpesvirus 8, Human/genetics, Host-Pathogen Interactions, Humans, Insulin/blood, Male, Middle Aged, Risk Factors, Secretory Pathway, Viral Load",

author = "Eric Lontchi-Yimagou and J{\'e}r{\^o}me Legoff and Jean-Louis Nguewa and Philippe Boudou and Balti, {Eric V} and Noubiap, {Jean J} and Vicky Kamwa and Barbara Atogho-Tiedeu and Marcel Azabji-Kenfack and Djahmeni, {Eric N} and Martine Etoa and Gaelle Lemdjo and Vanessa Balla and Dehayem, {Mesmin Y} and Fabienne Foufelle and Jean-Claude Mbanya and Jean-Francois Gautier and Eugene Sobngwi and Vicky Kamwa",

note = "{\textcopyright} 2018 Ruijin Hospital, Shanghai Jiaotong University School of Medicine and John Wiley & Sons Australia, Ltd.",

year = "2018",

month = nov,

doi = "10.1111/1753-0407.12777",

language = "English",

volume = "10",

pages = "866--873",

journal = "Journal of Diabetes",

issn = "1753-0393",

publisher = "Wiley-Blackwell",

number = "11",

}

Lontchi-Yimagou, E, Legoff, J, Nguewa, J-L, Boudou, P, Balti, EV, Noubiap, JJ, Kamwa, V, Atogho-Tiedeu, B, Azabji-Kenfack, M, Djahmeni, EN, Etoa, M, Lemdjo, G, Balla, V, Dehayem, MY, Foufelle, F, Mbanya, J-C, Gautier, J-F, Sobngwi, E & Kamwa, V 2018, 'Human herpesvirus 8 infection DNA positivity is associated with low insulin secretion: A case-control study in a sub-Saharan African population with diabetes', Journal of Diabetes, vol. 10, no. 11, pp. 866-873. https://doi.org/10.1111/1753-0407.12777

TY - JOUR

T1 - Human herpesvirus 8 infection DNA positivity is associated with low insulin secretion

T2 - A case-control study in a sub-Saharan African population with diabetes

AU - Lontchi-Yimagou, Eric

AU - Legoff, Jérôme

AU - Nguewa, Jean-Louis

AU - Boudou, Philippe

AU - Balti, Eric V

AU - Noubiap, Jean J

AU - Kamwa, Vicky

AU - Atogho-Tiedeu, Barbara

AU - Azabji-Kenfack, Marcel

AU - Djahmeni, Eric N

AU - Etoa, Martine

AU - Lemdjo, Gaelle

AU - Balla, Vanessa

AU - Dehayem, Mesmin Y

AU - Foufelle, Fabienne

AU - Mbanya, Jean-Claude

AU - Gautier, Jean-Francois

AU - Sobngwi, Eugene

AU - Kamwa, Vicky

PY - 2018/11

Y1 - 2018/11

N2 - BACKGROUND: Viruses have been considered potential triggers for the development of diabetes. This study assessed insulin secretion and insulin sensitivity in human herpesvirus 8 (HHV8)-infected and uninfected sub-Saharan African people with diabetes.METHODS: In all, 173 people with non-autoimmune diabetes were enrolled consecutively: 124 with type 2 diabetes mellitus (T2DM) and 49 with ketosis-prone diabetes (KPD) admitted in hyperglycemic crisis. Those with KPD were further subdivided into those with new-onset ketotic-phase KPD (n = 34) or non-ketotic phase KPD (n = 15). All participants were screened for HHV8-specific antibodies and genomic DNA. Blood samples were collected for analysis of fasting glucose, HbA1c, lipid profile, and C-peptide, with insulin resistance and secretion estimated by homeostasis model assessment.RESULTS: Among the 173 diabetic participants, 88 (50.9%) were positive for HHV8 antibodies (Ac-HHV8+), including 15 (8.7%) positive for HHV8 DNA (DNA-HHV8+). The seroprevalence of HHV8 was similar between T2DM (55.6%) and KPD (61.2%) subjects. Of those with and without ketotic-phase KPD, 35.3% and 46.7% were Ac-HHV8+, respectively. Body mass index was significantly in lower DNA-HHV8+ than DNA-HHV8- subjects. Low-density lipoprotein and total cholesterol were significantly higher, but C-peptide and homeostatic model assessment of β-cell function (HOMA-β) were significantly lower in DNA-HHV8+ than DNA-HHV8- participants. After excluding DNA-HHV8+ participants, triglyceride concentrations were significantly higher in Ac-HHV8+ (n = 73) than Ac-HHV8- (n = 85) subjects. In contrast, HOMA-β was significantly higher among Ac-HHV8+ than Ac-HHV8- participants.CONCLUSIONS: In the present study, HHV8 DNA positivity was associated with low insulin secretion in this sub-Saharan African diabetes population.

AB - BACKGROUND: Viruses have been considered potential triggers for the development of diabetes. This study assessed insulin secretion and insulin sensitivity in human herpesvirus 8 (HHV8)-infected and uninfected sub-Saharan African people with diabetes.METHODS: In all, 173 people with non-autoimmune diabetes were enrolled consecutively: 124 with type 2 diabetes mellitus (T2DM) and 49 with ketosis-prone diabetes (KPD) admitted in hyperglycemic crisis. Those with KPD were further subdivided into those with new-onset ketotic-phase KPD (n = 34) or non-ketotic phase KPD (n = 15). All participants were screened for HHV8-specific antibodies and genomic DNA. Blood samples were collected for analysis of fasting glucose, HbA1c, lipid profile, and C-peptide, with insulin resistance and secretion estimated by homeostasis model assessment.RESULTS: Among the 173 diabetic participants, 88 (50.9%) were positive for HHV8 antibodies (Ac-HHV8+), including 15 (8.7%) positive for HHV8 DNA (DNA-HHV8+). The seroprevalence of HHV8 was similar between T2DM (55.6%) and KPD (61.2%) subjects. Of those with and without ketotic-phase KPD, 35.3% and 46.7% were Ac-HHV8+, respectively. Body mass index was significantly in lower DNA-HHV8+ than DNA-HHV8- subjects. Low-density lipoprotein and total cholesterol were significantly higher, but C-peptide and homeostatic model assessment of β-cell function (HOMA-β) were significantly lower in DNA-HHV8+ than DNA-HHV8- participants. After excluding DNA-HHV8+ participants, triglyceride concentrations were significantly higher in Ac-HHV8+ (n = 73) than Ac-HHV8- (n = 85) subjects. In contrast, HOMA-β was significantly higher among Ac-HHV8+ than Ac-HHV8- participants.CONCLUSIONS: In the present study, HHV8 DNA positivity was associated with low insulin secretion in this sub-Saharan African diabetes population.

KW - Adult

KW - Biomarkers/blood

KW - Cameroon/epidemiology

KW - Case-Control Studies

KW - DNA, Viral/genetics

KW - Diabetes Mellitus/blood

KW - Female

KW - Herpesviridae Infections/blood

KW - Herpesvirus 8, Human/genetics

KW - Host-Pathogen Interactions

KW - Humans

KW - Insulin/blood

KW - Male

KW - Middle Aged

KW - Risk Factors

KW - Secretory Pathway

KW - Viral Load

U2 - 10.1111/1753-0407.12777

DO - 10.1111/1753-0407.12777

M3 - Article

C2 - 29707905

SN - 1753-0393

VL - 10

SP - 866

EP - 873

JO - Journal of Diabetes

JF - Journal of Diabetes

IS - 11

ER -

Human herpesvirus 8 infection DNA positivity is associated with low insulin secretion: A case-control study in a sub-Saharan African population with diabetes

Abstract

Bibliographical note

Keywords

UN SDGs

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