HSV-tk gene therapy for human renal cell carcinoma in nude mice

K Pulkkanen; JJ Parkkinen; JM Laukkanen; MI Kettunen; K Tyynela; Risto Kauppinen; MY Ala-Opas; S Yla-Herttuala

doi:10.1038/sj.cgt.7700342

HSV-tk gene therapy for human renal cell carcinoma in nude mice

K Pulkkanen, JJ Parkkinen, JM Laukkanen, MI Kettunen, K Tyynela, Risto Kauppinen, MY Ala-Opas, S Yla-Herttuala

Sport, Exercise and Rehabilitation Sciences

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Abstract

We have treated Caki-2 human renal cell carcinoma in vivo using herpes simplex virus thymidine kinase (HSV-tk) gene therapy. Both stably transduced Caki-2 tumors, generated using retrovirus-mediated ex vivo HSV-tk gene transfer and direct intratumoral adenovirus-mediated HSV-tk gene transfer of wild type tumors, were tested. Similar treatments with LacZ containing retro- and adenoviruses were used as controls. The outcome was evaluated by imaging the tumors before and after the treatment with magnetic resonance imaging, and using histology, immunocytochemistry, and survival analysis. When implanted orthotopically into nude mouse kidneys, Caki-2 cells formed reproducible cystic papillary kidney carcinomas. In vivo magnetic resonance imaging provided an important tool for the evaluation of tumor growth. Transduction efficiency of wild-type tumors in vivo with adeno-LacZ was 22+/-14%. Significant tumor regression was achieved with direct intratumoral adeno-HSV-tk transduction followed by intraperitoneal ganciclovir (GCV) (P

Original language	English
Pages (from-to)	529-536
Number of pages	8
Journal	Cancer Gene Therapy
Volume	8
DOIs	https://doi.org/10.1038/sj.cgt.7700342
Publication status	Published - 1 Jul 2001

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title = "HSV-tk gene therapy for human renal cell carcinoma in nude mice",

abstract = "We have treated Caki-2 human renal cell carcinoma in vivo using herpes simplex virus thymidine kinase (HSV-tk) gene therapy. Both stably transduced Caki-2 tumors, generated using retrovirus-mediated ex vivo HSV-tk gene transfer and direct intratumoral adenovirus-mediated HSV-tk gene transfer of wild type tumors, were tested. Similar treatments with LacZ containing retro- and adenoviruses were used as controls. The outcome was evaluated by imaging the tumors before and after the treatment with magnetic resonance imaging, and using histology, immunocytochemistry, and survival analysis. When implanted orthotopically into nude mouse kidneys, Caki-2 cells formed reproducible cystic papillary kidney carcinomas. In vivo magnetic resonance imaging provided an important tool for the evaluation of tumor growth. Transduction efficiency of wild-type tumors in vivo with adeno-LacZ was 22+/-14%. Significant tumor regression was achieved with direct intratumoral adeno-HSV-tk transduction followed by intraperitoneal ganciclovir (GCV) (P",

author = "K Pulkkanen and JJ Parkkinen and JM Laukkanen and MI Kettunen and K Tyynela and Risto Kauppinen and MY Ala-Opas and S Yla-Herttuala",

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doi = "10.1038/sj.cgt.7700342",

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T1 - HSV-tk gene therapy for human renal cell carcinoma in nude mice

AU - Pulkkanen, K

AU - Parkkinen, JJ

AU - Laukkanen, JM

AU - Kettunen, MI

AU - Tyynela, K

AU - Kauppinen, Risto

AU - Ala-Opas, MY

AU - Yla-Herttuala, S

PY - 2001/7/1

Y1 - 2001/7/1

N2 - We have treated Caki-2 human renal cell carcinoma in vivo using herpes simplex virus thymidine kinase (HSV-tk) gene therapy. Both stably transduced Caki-2 tumors, generated using retrovirus-mediated ex vivo HSV-tk gene transfer and direct intratumoral adenovirus-mediated HSV-tk gene transfer of wild type tumors, were tested. Similar treatments with LacZ containing retro- and adenoviruses were used as controls. The outcome was evaluated by imaging the tumors before and after the treatment with magnetic resonance imaging, and using histology, immunocytochemistry, and survival analysis. When implanted orthotopically into nude mouse kidneys, Caki-2 cells formed reproducible cystic papillary kidney carcinomas. In vivo magnetic resonance imaging provided an important tool for the evaluation of tumor growth. Transduction efficiency of wild-type tumors in vivo with adeno-LacZ was 22+/-14%. Significant tumor regression was achieved with direct intratumoral adeno-HSV-tk transduction followed by intraperitoneal ganciclovir (GCV) (P

AB - We have treated Caki-2 human renal cell carcinoma in vivo using herpes simplex virus thymidine kinase (HSV-tk) gene therapy. Both stably transduced Caki-2 tumors, generated using retrovirus-mediated ex vivo HSV-tk gene transfer and direct intratumoral adenovirus-mediated HSV-tk gene transfer of wild type tumors, were tested. Similar treatments with LacZ containing retro- and adenoviruses were used as controls. The outcome was evaluated by imaging the tumors before and after the treatment with magnetic resonance imaging, and using histology, immunocytochemistry, and survival analysis. When implanted orthotopically into nude mouse kidneys, Caki-2 cells formed reproducible cystic papillary kidney carcinomas. In vivo magnetic resonance imaging provided an important tool for the evaluation of tumor growth. Transduction efficiency of wild-type tumors in vivo with adeno-LacZ was 22+/-14%. Significant tumor regression was achieved with direct intratumoral adeno-HSV-tk transduction followed by intraperitoneal ganciclovir (GCV) (P

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DO - 10.1038/sj.cgt.7700342

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VL - 8

SP - 529

EP - 536

JO - Cancer Gene Therapy

JF - Cancer Gene Therapy

ER -

HSV-tk gene therapy for human renal cell carcinoma in nude mice

Abstract

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