High-throughput whole-genome sequencing to dissect the epidemiology of Acinetobacter baumannii isolates from a hospital outbreak.

T Lewis; Nicholas Loman; Lewis Bingle; P Jumaa; GM Weinstock; D Mortiboy; Mark Pallen

doi:10.1016/j.jhin.2010.01.012

High-throughput whole-genome sequencing to dissect the epidemiology of Acinetobacter baumannii isolates from a hospital outbreak.

T Lewis, Nicholas Loman, Lewis Bingle, P Jumaa, GM Weinstock, D Mortiboy, Mark Pallen

Biosciences

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Abstract

Shared care of military and civilian patients has resulted in transmission of multidrug-resistant Acinetobacter baumannii (MDR-Aci) from military casualties to civilians. Current typing technologies have been useful in revealing relationships between isolates of A. baumannii but they are unable to resolve differences between closely related isolates from small-scale outbreaks, where chains of transmission are often unclear. In a recent hospital outbreak in Birmingham, six patients were colonised with MDR-Aci isolates indistinguishable using standard techniques. We used whole-genome sequencing to identify single nucleotide polymorphisms in these isolates, allowing us to discriminate between alternative epidemiological hypotheses in this setting.

Original language	English
Pages (from-to)	37-41
Number of pages	5
Journal	The Journal of hospital infection
Volume	75
Issue number	1
DOIs	https://doi.org/10.1016/j.jhin.2010.01.012
Publication status	Published - 1 May 2010

Keywords

Acinetobacter baumannii
Single nucleotide polymorphism
Epidemiology
Multidrug resistance
454 pyrosequencing
Whole-genome sequencing

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10.1016/j.jhin.2010.01.012

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abstract = "Shared care of military and civilian patients has resulted in transmission of multidrug-resistant Acinetobacter baumannii (MDR-Aci) from military casualties to civilians. Current typing technologies have been useful in revealing relationships between isolates of A. baumannii but they are unable to resolve differences between closely related isolates from small-scale outbreaks, where chains of transmission are often unclear. In a recent hospital outbreak in Birmingham, six patients were colonised with MDR-Aci isolates indistinguishable using standard techniques. We used whole-genome sequencing to identify single nucleotide polymorphisms in these isolates, allowing us to discriminate between alternative epidemiological hypotheses in this setting.",

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AU - Bingle, Lewis

AU - Jumaa, P

AU - Weinstock, GM

AU - Mortiboy, D

AU - Pallen, Mark

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AB - Shared care of military and civilian patients has resulted in transmission of multidrug-resistant Acinetobacter baumannii (MDR-Aci) from military casualties to civilians. Current typing technologies have been useful in revealing relationships between isolates of A. baumannii but they are unable to resolve differences between closely related isolates from small-scale outbreaks, where chains of transmission are often unclear. In a recent hospital outbreak in Birmingham, six patients were colonised with MDR-Aci isolates indistinguishable using standard techniques. We used whole-genome sequencing to identify single nucleotide polymorphisms in these isolates, allowing us to discriminate between alternative epidemiological hypotheses in this setting.

KW - Acinetobacter baumannii

KW - Single nucleotide polymorphism

KW - Epidemiology

KW - Multidrug resistance

KW - 454 pyrosequencing

KW - Whole-genome sequencing

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JO - The Journal of hospital infection

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ER -

High-throughput whole-genome sequencing to dissect the epidemiology of Acinetobacter baumannii isolates from a hospital outbreak.

Abstract

Keywords

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