Projects per year
Abstract
Recent studies suggest that vitamin D-deficiency is linked to increased risk of common human health problems. To define vitamin D 'status' most routine analytical methods quantify one particular vitamin D metabolite, 25-hydroxyvitamin D3 (25OHD3). However, vitamin D is characterized by complex metabolic pathways, and simultaneous measurement of multiple vitamin D metabolites may provide a more accurate interpretation of vitamin D status. To address this we developed a high-throughput liquid chromatography-tandem mass spectrometry (LC-MS/MS) method to analyse multiple vitamin D analytes, with particular emphasis on the separation of epimer metabolites. A supportive liquid-liquid extraction (SLE) and LC-MS/MS method was developed to quantify 10 vitamin D metabolites as well as separation of an interfering 7α-hydroxy-4-cholesten-3-one (7αC4) isobar (precursor of bile acid), and validated by analysis of human serum samples. In a cohort of 116 healthy subjects, circulating concentrations of 25-hydroxyvitamin D3 (25OHD3), 3-epi-25-hydroxyvitamin D3 (3-epi-25OHD3), 24,25-dihydroxyvitamin D3 (24R,25(OH)2D3), 1,25-dihydroxyvitamin D3 (1α,25(OH)2D3), and 25-hydroxyvitamin D2 (25OHD2) were quantifiable using 220μL of serum, with 25OHD3 and 24R,25(OH)2D3 showing significant seasonal variations. This high-throughput LC-MS/MS method provides a novel strategy for assessing the impact of vitamin D on human health and disease.
Original language | English |
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Pages (from-to) | 56-63 |
Number of pages | 8 |
Journal | Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences |
Volume | 1014 |
Early online date | 2 Feb 2016 |
DOIs | |
Publication status | Published - Mar 2016 |
Keywords
- LC–MS/MS
- Vitamin D
- Method validation
- Chiral separation
- Serum analysis
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Dive into the research topics of 'High throughput LC-MS/MS method for the simultaneous analysis of multiple vitamin D analytes in serum'. Together they form a unique fingerprint.Projects
- 5 Finished
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Assessing the Therapeutic Efficacy of an 11Beta-Hydroxysteroid Dehydrogenase Type 1 Inhibitor (AZD4017) in Idiopathic Intracranial Hypertension (IIH)
Sinclair, A., Tomlinson, J. & Stewart, P.
12/08/13 → 11/08/17
Project: Research Councils
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Centre for Musculoskeletal Ageing Research (linked to 18289 & 19482)
Lord, J., Buckley, C., Duda, J., Dunn, W., Miall, C. & Greig, C.
1/08/12 → 31/07/17
Project: Research Councils
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Steroid Profiling as a Biomarker Tool in the Diagnosis and Monitoring of Adrenal Tumours
Arlt, W. & Stewart, P.
2/03/09 → 29/02/12
Project: Research Councils
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Selective Inhibition of 11beta-Hydroxysteroid Dehydrogenase Type 1: A Novel Treatment for the Metabolic Syndrome
Tomlinson, J. & Stewart, P.
1/10/06 → 30/09/09
Project: Research Councils
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Pre-receptor Metabolism and the Control of Hormone Action
Stewart, P.
1/12/02 → 31/05/08
Project: Research Councils