High resolution optical mapping of cardiac electrophysiology in pre-clinical models

Christopher O’Shea; James Winter; S. Nashitha Kabir; Molly O’Reilly; Simon P. Wells; Olivia Baines; Laura C. Sommerfeld; Joao Correia; Ming Lei; Paulus Kirchhof; Andrew P. Holmes; Larissa Fabritz; Kashif Rajpoot; Davor Pavlovic

doi:10.1038/s41597-022-01253-1

High resolution optical mapping of cardiac electrophysiology in pre-clinical models

Christopher O’Shea^*, James Winter, S. Nashitha Kabir, Molly O’Reilly, Simon P. Wells, Olivia Baines, Laura C. Sommerfeld, Joao Correia, Ming Lei, Paulus Kirchhof, Andrew P. Holmes, Larissa Fabritz, Kashif Rajpoot, Davor Pavlovic

^*Corresponding author for this work

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Abstract

Optical mapping of animal models is a widely used technique in pre-clinical cardiac research. It has several advantages over other methods, including higher spatial resolution, contactless recording and direct visualisation of action potentials and calcium transients. Optical mapping enables simultaneous study of action potential and calcium transient morphology, conduction dynamics, regional heterogeneity, restitution and arrhythmogenesis. In this dataset, we have optically mapped Langendorff perfused isolated whole hearts (mouse and guinea pig) and superfused isolated atria (mouse). Raw datasets (consisting of over 400 files) can be combined with open-source software for processing and analysis. We have generated a comprehensive post-processed dataset characterising the baseline cardiac electrophysiology in these widely used pre-clinical models. This dataset also provides reference information detailing the effect of heart rate, clinically used anti-arrhythmic drugs, ischaemia-reperfusion and sympathetic nervous stimulation on cardiac electrophysiology. The effects of these interventions can be studied in a global or regional manner, enabling new insights into the prevention and initiation of arrhythmia.

Original language	English
Article number	135
Number of pages	12
Journal	Scientific Data
Volume	9
Issue number	1
Early online date	31 Mar 2022
DOIs	https://doi.org/10.1038/s41597-022-01253-1
Publication status	Published - Dec 2022

Bibliographical note

Funding Information:
This work was funded by the Wellcome Trust (Sir Henry Wellcome Fellowship 221650/Z/20/Z to COS, Seed Award Grant 109604/Z/15/Z to DP), EPSRC (Sci-Phy-4-Health Centre for Doctoral Training studentship L016346 to DP, KR and LF), the British Heart Foundation (PG/17/55/33087, RG/17/15/33106 to DP, PG/17/30/32961 to AH, Accelerator Award AA/18/2/34218 to Institute of Cardiovascular Sciences, FS/13/43/30324 to PK and LF ), EU CATCH ME (633196 to PK and LF), MAESTRIA (965286 to LF), and the Leducq Foundation (PK).

Publisher Copyright:
© 2022, The Author(s).

ASJC Scopus subject areas

Statistics and Probability
Information Systems
Education
Computer Science Applications
Statistics, Probability and Uncertainty
Library and Information Sciences

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1 Active
5 Finished

BHF Research Accelerator Award
Bicknell, R., Watson, S., Rainger, E., Senis, Y., Fabritz, L. & Kirchhof, P.
British Heart Foundation
1/04/19 → 30/09/24
Project: Research
Cardiotonic steroids in patients with atrial fibrillation and heart failure: Quantification, functional effects and personalisation of digoxin therapy
Arlt, W., Pavlovic, D., Taylor, A., Kotecha, D. & Kirchhof, P.
British Heart Foundation
1/06/18 → 30/05/21
Project: Research
Impact of chronic intermittent hypoxia on atrial resting membrane potential and Na 1.5 channel function: A new mechanism for causing atrial fibrillation
Kirchhof, P. & Holmes, A.
British Heart Foundation
1/10/17 → 18/07/21
Project: Research
Examining the role of endogenous cardiotonic steriods in cardiovascular disease development.
Pavlovic, D.
THE WELLCOME TRUST
1/04/16 → 31/03/18
Project: Research
H2020_COLLAB_CATCH ME_(LEAD)
Kirchhof, P., Fabritz, L., Hemming, K. & Deeks, J.
European Commission
1/05/15 → 30/04/19
Project: EU
Understanding the function of PITX2 in the adult left atrium
Kirchhof, P. & Fabritz, L.
British Heart Foundation
4/11/13 → 3/11/18
Project: Research

Cite this

O’Shea, C., Winter, J., Kabir, S. N., O’Reilly, M., Wells, S. P., Baines, O., Sommerfeld, L. C., Correia, J., Lei, M., Kirchhof, P., Holmes, A. P., Fabritz, L., Rajpoot, K., & Pavlovic, D. (2022). High resolution optical mapping of cardiac electrophysiology in pre-clinical models. Scientific Data, 9(1), Article 135. Advance online publication. https://doi.org/10.1038/s41597-022-01253-1

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abstract = "Optical mapping of animal models is a widely used technique in pre-clinical cardiac research. It has several advantages over other methods, including higher spatial resolution, contactless recording and direct visualisation of action potentials and calcium transients. Optical mapping enables simultaneous study of action potential and calcium transient morphology, conduction dynamics, regional heterogeneity, restitution and arrhythmogenesis. In this dataset, we have optically mapped Langendorff perfused isolated whole hearts (mouse and guinea pig) and superfused isolated atria (mouse). Raw datasets (consisting of over 400 files) can be combined with open-source software for processing and analysis. We have generated a comprehensive post-processed dataset characterising the baseline cardiac electrophysiology in these widely used pre-clinical models. This dataset also provides reference information detailing the effect of heart rate, clinically used anti-arrhythmic drugs, ischaemia-reperfusion and sympathetic nervous stimulation on cardiac electrophysiology. The effects of these interventions can be studied in a global or regional manner, enabling new insights into the prevention and initiation of arrhythmia.",

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note = "Funding Information: This work was funded by the Wellcome Trust (Sir Henry Wellcome Fellowship 221650/Z/20/Z to COS, Seed Award Grant 109604/Z/15/Z to DP), EPSRC (Sci-Phy-4-Health Centre for Doctoral Training studentship L016346 to DP, KR and LF), the British Heart Foundation (PG/17/55/33087, RG/17/15/33106 to DP, PG/17/30/32961 to AH, Accelerator Award AA/18/2/34218 to Institute of Cardiovascular Sciences, FS/13/43/30324 to PK and LF ), EU CATCH ME (633196 to PK and LF), MAESTRIA (965286 to LF), and the Leducq Foundation (PK). Publisher Copyright: {\textcopyright} 2022, The Author(s).",

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AU - Winter, James

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AU - Wells, Simon P.

AU - Baines, Olivia

AU - Sommerfeld, Laura C.

AU - Correia, Joao

AU - Lei, Ming

AU - Kirchhof, Paulus

AU - Holmes, Andrew P.

AU - Fabritz, Larissa

AU - Rajpoot, Kashif

AU - Pavlovic, Davor

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N2 - Optical mapping of animal models is a widely used technique in pre-clinical cardiac research. It has several advantages over other methods, including higher spatial resolution, contactless recording and direct visualisation of action potentials and calcium transients. Optical mapping enables simultaneous study of action potential and calcium transient morphology, conduction dynamics, regional heterogeneity, restitution and arrhythmogenesis. In this dataset, we have optically mapped Langendorff perfused isolated whole hearts (mouse and guinea pig) and superfused isolated atria (mouse). Raw datasets (consisting of over 400 files) can be combined with open-source software for processing and analysis. We have generated a comprehensive post-processed dataset characterising the baseline cardiac electrophysiology in these widely used pre-clinical models. This dataset also provides reference information detailing the effect of heart rate, clinically used anti-arrhythmic drugs, ischaemia-reperfusion and sympathetic nervous stimulation on cardiac electrophysiology. The effects of these interventions can be studied in a global or regional manner, enabling new insights into the prevention and initiation of arrhythmia.

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High resolution optical mapping of cardiac electrophysiology in pre-clinical models

Abstract

Bibliographical note

ASJC Scopus subject areas

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Projects

BHF Research Accelerator Award

Cardiotonic steroids in patients with atrial fibrillation and heart failure: Quantification, functional effects and personalisation of digoxin therapy

Impact of chronic intermittent hypoxia on atrial resting membrane potential and Na 1.5 channel function: A new mechanism for causing atrial fibrillation

Examining the role of endogenous cardiotonic steriods in cardiovascular disease development.

H2020_COLLAB_CATCH ME_(LEAD)

Understanding the function of PITX2 in the adult left atrium

Cite this