Hepatoma cell density promotes Claudin-1 and scavenger receptor BI expression and hepatitis C virus internalization

AK Schwarz, Joseph Grove, Ke Hu, Christopher Mee, Peter Balfe, Jane McKeating

Research output: Contribution to journalArticlepeer-review

35 Citations (Scopus)
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Abstract

Hepatitis C virus (HCV) entry occurs via a pH- and clathrin-dependent endocytic pathway and requires a number of cellular factors, including CD81, the tight-junction proteins claudin 1 (CLDN1) and occludin, and scavenger receptor class B member I (SR-BI). HCV tropism is restricted to the liver, where hepatocytes are tightly packed. Here, we demonstrate that SR-BI and CLDN1 expression is modulated in confluent human hepatoma cells, with both receptors being enriched at cell-cell junctions. Cellular contact increased HCV pseudoparticle (HCVpp) and HCV particle (HCVcc) infection and accelerated the internalization of cell-bound HCVcc, suggesting that the cell contact modulation of receptor levels may facilitate the assembly of receptor complexes required for virus internalization. CLDN1 overexpression in subconfluent cells was unable to recapitulate this effect, whereas increased SR-BI expression enhanced HCVpp entry and HCVcc internalization, demonstrating a rate-limiting role for SR-BI in HCV internalization.
Original languageEnglish
Pages (from-to)12407-12414
JournalJournal of virology
Volume83
Issue number23
Early online date23 Sep 2009
DOIs
Publication statusPublished - 1 Dec 2009

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