Hepatitis C virus infection of cholangiocarcinoma cell lines

N. F. Fletcher, E. A. Humphreys, E. Jennings, W. Osburn, Samantha Lissauer, G. K. Wilson, S. C. Van Ijzendoorn, T. F. Baumert, P. Balfe, S. Afford, J. Mckeating

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6 Citations (Scopus)
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Hepatitis C virus (HCV) infects the liver and hepatocytes are the major cell type supporting viral replication. Hepatocytes and cholangiocytes derive from a common hepatic progenitor cell that proliferates during inflammatory conditions, raising the possibility that cholangiocytes may support HCV replication and contribute to the hepatic reservoir. We screened cholangiocytes along with a panel of cholangiocarcinoma-derived cell lines for their ability to support HCV entry and replication. While primary cholangiocytes were refractory to infection and lacked expression of several entry factors, two cholangiocarcinoma lines, CC-LP-1 and Sk-ChA-1, supported efficient HCV entry; furthermore, Sk-ChA-1 cells supported full virus replication. In vivo cholangiocarcinomas expressed all of the essential HCV entry factors; however, cholangiocytes adjacent to the tumor and in normal tissue showed a similar pattern of receptor expression to ex vivo isolated cholangiocytes, lacking SR-BI expression, explaining their inability to support infection. This study provides the first report that HCV can infect cholangiocarcinoma cells and suggests that these heterogeneous tumors may provide a reservoir for HCV replication in vivo.
Original languageEnglish
Pages (from-to)1380-1388
Number of pages8
JournalJournal of General Virology
Issue number6
Early online date20 Feb 2015
Publication statusPublished - Jun 2015


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