Projects per year
Abstract
Hepatitis C virus (HCV) infects the liver and hepatocytes are the major cell type supporting viral replication. Hepatocytes and cholangiocytes derive from a common hepatic progenitor cell that proliferates during inflammatory conditions, raising the possibility that cholangiocytes may support HCV replication and contribute to the hepatic reservoir. We screened cholangiocytes along with a panel of cholangiocarcinoma-derived cell lines for their ability to support HCV entry and replication. While primary cholangiocytes were refractory to infection and lacked expression of several entry factors, two cholangiocarcinoma lines, CC-LP-1 and Sk-ChA-1, supported efficient HCV entry; furthermore, Sk-ChA-1 cells supported full virus replication. In vivo cholangiocarcinomas expressed all of the essential HCV entry factors; however, cholangiocytes adjacent to the tumor and in normal tissue showed a similar pattern of receptor expression to ex vivo isolated cholangiocytes, lacking SR-BI expression, explaining their inability to support infection. This study provides the first report that HCV can infect cholangiocarcinoma cells and suggests that these heterogeneous tumors may provide a reservoir for HCV replication in vivo.
Original language | English |
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Pages (from-to) | 1380-1388 |
Number of pages | 8 |
Journal | Journal of General Virology |
Volume | 96 |
Issue number | 6 |
Early online date | 20 Feb 2015 |
DOIs | |
Publication status | Published - Jun 2015 |
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Dive into the research topics of 'Hepatitis C virus infection of cholangiocarcinoma cell lines'. Together they form a unique fingerprint.Projects
- 2 Finished
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The Role of Hepatitis C Virus Glycoprotein-Receptor Polymorphism in Viral Pathogenesis
McKeating, J. (Principal Investigator)
1/01/12 → 30/06/17
Project: Research Councils
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Mechanisms of Hepatitis C Virus Induced Hepatocyte Injury
McKeating, J. (Principal Investigator) & Balfe, P. (Co-Investigator)
1/10/09 → 30/09/12
Project: Research Councils