TY - JOUR
T1 - Hepatitis C virus glycoproteins mediate pH-dependent cell entry of pseudotyped retroviral particles
AU - Hsu, M
AU - Zhang, J
AU - Flint, M
AU - Logvinoff, C
AU - Cheng-Mayrer, C
AU - Rice, CM
AU - McKeating, Jane
PY - 2003/6/2
Y1 - 2003/6/2
N2 - HIV pseudotypes bearing native hepatitis C virus (HCV) glycoproteins (strain H and Con1) are infectious for the human hepatoma cell lines Huh-7 and PLC/PR5. Infectivity depends on coexpression of both E1 and E2 glycoproteins, is pH-dependent, and can be neutralized by mAbs mapping to amino acids 412-447 within E2. Cell-surface expression of one or all of the candidate receptor molecules (CD81, low-density lipoprotein receptor, scavenger receptor class B type 1, and dendritic cell-specific intercellular adhesion molecule 3 grabbing nonintegrin) failed to confer permissivity to HIV-HCV pseudotype infection. However, HIV-HCV pseudotype infectivity was inhibited by a recombinant soluble form of CD81 and a mAb specific for CD81, suggesting that CD81 may be a component of a receptor complex.
AB - HIV pseudotypes bearing native hepatitis C virus (HCV) glycoproteins (strain H and Con1) are infectious for the human hepatoma cell lines Huh-7 and PLC/PR5. Infectivity depends on coexpression of both E1 and E2 glycoproteins, is pH-dependent, and can be neutralized by mAbs mapping to amino acids 412-447 within E2. Cell-surface expression of one or all of the candidate receptor molecules (CD81, low-density lipoprotein receptor, scavenger receptor class B type 1, and dendritic cell-specific intercellular adhesion molecule 3 grabbing nonintegrin) failed to confer permissivity to HIV-HCV pseudotype infection. However, HIV-HCV pseudotype infectivity was inhibited by a recombinant soluble form of CD81 and a mAb specific for CD81, suggesting that CD81 may be a component of a receptor complex.
UR - http://www.scopus.com/inward/record.url?scp=0038471344&partnerID=8YFLogxK
U2 - 10.1073/pnas.0832180100
DO - 10.1073/pnas.0832180100
M3 - Article
C2 - 12761383
SN - 1091-6490
VL - 100
SP - 7271
EP - 7276
JO - National Academy of Sciences. Proceedings
JF - National Academy of Sciences. Proceedings
ER -