TY - JOUR
T1 - Genome-wide identification of binding sites for the nitric oxide-sensitive transcriptional regulator NsrR
AU - Efromovich, S
AU - Grainger, David
AU - Bodenmiller, D
AU - Spiro, S
PY - 2008/1/1
Y1 - 2008/1/1
N2 - NsrR is a nitric oxide-sensitive regulator of transcription. In Escherichia coli, NsrR is a repressor of the hmp gene encoding the flavohemoglobin that detoxifies nitric oxide. Three other transcription units (ytfE, ygbA, and hcp-hcr) are known to be subject to regulation by NsrR. This chapter describes experimental and statistical protocols used to identify NsrR-binding sites in the E. colichromosome using chromatin immunoprecipitation and microarray analysis. The methods are applicable, with suitable modifications, to any regulatory protein and any organism.
AB - NsrR is a nitric oxide-sensitive regulator of transcription. In Escherichia coli, NsrR is a repressor of the hmp gene encoding the flavohemoglobin that detoxifies nitric oxide. Three other transcription units (ytfE, ygbA, and hcp-hcr) are known to be subject to regulation by NsrR. This chapter describes experimental and statistical protocols used to identify NsrR-binding sites in the E. colichromosome using chromatin immunoprecipitation and microarray analysis. The methods are applicable, with suitable modifications, to any regulatory protein and any organism.
U2 - 10.1016/S0076-6879(07)37012-2
DO - 10.1016/S0076-6879(07)37012-2
M3 - Review article
C2 - 18433631
VL - 437
SP - 211
EP - 233
JO - Methods in Enzymology
JF - Methods in Enzymology
ER -